Directed evolution of Clec12a-binding peptides using mammalian-expressed recombinant protein.

Yoshirou Kawaguchi, Md Samiul Islam, Mina Yokoyama, Takanatsu Hosokawa, Misuzu Nakaya, Noriho Kamiya, Masahiro Goto

Abstract read

In one paragraph

Article in Biochemistry and biophysics reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yoshirou KawaguchiDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Md Samiul IslamDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Mina YokoyamaDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Takanatsu HosokawaGraduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Misuzu NakayaDepartment of Applied Chemistry, School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Noriho KamiyaDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Masahiro GotoDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clec12a is an immunoregulatory C-type lectin receptor expressed on dendritic cells as well as other myeloid cells, and involved in maintaining immune homeostasis. Unlike many other C-type lectin receptors that promote immune activation, Clec12a transmits inhibitory signals through an immunoreceptor tyrosine-based inhibitory motif, thereby contributing to immune regulation. Although the extracellular domain of murine Clec12a has been produced in previous studies, its suitability as a functional bait for directed evolution-based ligand discovery has not been established, nor have quantitative yield and scalability been reported. Here, we established a mammalian expression system that reproducibly yields ∼80 mg/L of the mClec12a extracellular domain, providing material of sufficient quality and quantity for directed evolution of peptide binders. Incorporation of an interleukin-2 signal peptide enabled efficient secretion from Expi293 cells, and the purified protein retained

Indexed as

Clec12aDirected evolutionMammalian expressionPeptide binder screeningRibosome display

Identifiers

PMID41799695

DOI10.1016/j.bbrep.2026.102531

PMCPMC12966690

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.