C-terminal Fragment Generated by HOIL-1 Cleavage Suppresses Inflammatory Responses of Myeloid Cells to Alleviate Colitis.

Rationale: Deciphering the molecular consequences of protein cleavage in inflammatory signaling is vital for defining the mechanisms of intestinal autoinflammation and identifying new therapeutic targets for inflammatory bowel disease (IBD). While it was previously established that HOIL-1 cleavage by MALT1 negatively regulates NF-κB activation and inflammatory responses Methods: To investigate the impact of HOIL-1 cleavage on intestinal inflammation, the global and myeloid-specific transgenic mouse models with uncleavable HOIL-1 (lacking C-HOIL-1) were established, and their disease phenotypes and immune profiles were characterized under DSS-induced colitis. Genetically engineered THP-1 monocytic cells expressing uncleavable HOIL-1 and C-HOIL-1 were constructed to elucidate the molecular mechanisms of C-HOIL-1 in regulating inflammatory signaling. Finally, Lenti-C-HOIL-1 was delivered to the colon of wild-type mice via enema to evaluate the therapeutic potential of C-HOIL-1 in controlling intestinal inflammation. Results: Mice with uncleavable HOIL-1 (lacking C-HOIL-1) present a more severe disease phenotype in DSS-induced colitis; specifically, the infiltration of inflammatory monocytes, M1-type macrophages, and neutrophils is significantly elevated in the colon. Mechanistically, we discover that C-HOIL-1 has novel biological functions in i) inhibiting NF-κB signaling, ii) interacting with STAT1 to down-regulate STAT1-mediated inflammatory signaling, and iii) up-regulating Conclusions: Our results define the pathophysiological role of HOIL-1 cleavage in colitis, and unveil new functions of C-HOIL-1 in regulating myeloid inflammatory responses. These findings provide a potential therapeutic strategy for controlling gut inflammation in IBD.