ArticleJournal of the Endocrine Society2026
Insulin receptor trafficking and interactions in muscle cells.
Article in Journal of the Endocrine Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Insulin receptor trafficking and interactions in muscle cells.Journal of the Endocrine Society · 2026Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Context: Insulin action is critical for energy homeostasis and its dysfunction in muscle cells is associated with type 2 diabetes. Insulin receptor (INSR) internalization and cell-surface dynamics at rest and during insulin exposure are incompletely understood in muscle cells. Objective: We aimed to characterized the INSR dynamics and interactions in muscle. Methods: We applied inter-domain tagged INSR, microscopy, immunoprecipitation, mass spectrometry, and AlphaFold multimer to comprehensively profile INSR internalization and interactions with or without insulin stimulation. Results: Using surface labeling and live-cell imaging, we observed robust basal internalization of INSR in C2C12 myoblasts, without an effect of added insulin. Mass spectrometry using INSR knockout cells as controls identified high-confidence binding partners, including proteins associated with internalization. We confirmed known interactors, including insulin-like growth factor 1 receptor, and also identified underappreciated INSR-binding factors, such as annexin A2. AlphaFold multimer analysis predicted potential INSR-binding sites of these proteins. Protein-protein interaction network mapping suggested links between INSR and caveolin-mediated endocytosis. INSR interacted with both caveolin and clathrin heavy chain (CLTC) in mouse skeletal muscle and C2C12 myoblasts. Whole-cell 2-dimensional super-resolution imaging revealed that high levels of insulin (20 nM) increased INSR colocalization with caveolin-1 (CAV1) but decreased its colocalization with CLTC. Single-particle tracking confirmed the colocalization of cell-surface INSR with both overexpressed CAV1-mRFP (monomeric red fluorescent protein) and CLTC-mRFP. INSR tracks that colocalized with CAV1 exhibited longer radii and lifetimes, regardless of insulin exposure, compared with noncolocalized tracks, whereas insulin further increased the lifetime of INSR/CLTC-colocalized tracks. Conclusion: Overall, these data suggest that muscle cells utilize both CAV1- and CLTC-dependent pathways for INSR mobilization and internalization.
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