Evidence map›Paper›PMID 41798072›Full record

ReviewJournal of pharmaceutical analysis2026

Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review.

David S Hage, Sadia Sharmeen, B K Sajeeb, Harshana Olupathage, Md Masudur Rahman, Isaac Kyei, Samiul Alim, Nigar Sultana Pinky

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of pharmaceutical analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

David S HageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Sadia SharmeenDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
B K SajeebDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Harshana OlupathageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Md Masudur RahmanDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Isaac KyeiDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Samiul AlimDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
Nigar Sultana PinkyDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The characterization of drug-target interactions is a key component of drug discovery, testing, and development. Affinity chromatography is one approach that can be used for this type of analysis. For instance, this may be done by using an immobilized target as a stationary phase and a drug as the applied solute. This review will discuss the various ways in which affinity chromatographic methods have been used to examine drug-target interactions, with an emphasis on high-performance methods. The general principles of this approach and factors to consider in its use for drug-target interaction analysis will first be examined. Methods based on zonal elution or frontal analysis for binding and competition studies will then be discussed. Various techniques for kinetic studies will next be considered, along with approaches that employ secondary binding agents and hybrid techniques. In each case, the general principles and theory of an approach will be given along with examples of its use in drug-target interaction studies. Advantages or limitations of each approach will be provided as well. This information should make it possible in the future to extend these techniques to other drug-target systems of interest in biomedical research and drug testing or development.

Indexed as

Affinity chromatographyBiointeraction analysisDrug-protein bindingDrug-target interactionsHigh-performance affinity chromatographyKinetic analysis

Identifiers

PMID41798072
PMCPMC12966675

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.