Evidence map›Paper›PMID 41795275›Full record

ArticlePoultry science2026

Effects of resveratrol in a chronic stress model of layer chicks: insights from transcriptomics, metabolomics, and network pharmacology.

Qingqing Chang, Haiyue Gong, Liying Pan, Jiaqi Zhang, Jingyi Zhang, Farooque Laghari, Jianhong Li, Jun Bao, Runxiang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Qingqing ChangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Haiyue GongCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Liying PanCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Jiaqi ZhangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Jingyi ZhangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Farooque LaghariCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Jianhong LiCollege of Life Science, Northeast Agricultural University, Harbin 150030, China.
Jun BaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China.
Runxiang ZhangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China. Electronic address: zhangrunxiang@neau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic stress induces neuroinflammation, hypothalamic dysfunction, and behavioral abnormalities, contributing to depressive-like states. The function of resveratrol (RES), a natural polyphenolic compound with neuroprotective and antidepressant properties, remains underexplored in avian models. This study evaluated the effects of RES on chronic unpredictable mild stress (CUMS), induced depressive-like behaviors in layer chicks and elucidated underlying mechanisms through integrated multi-omics approaches. Behavioral assessments, including open field test (OFT), tonic immobility (TI), and attention bias test (ABT), confirmed that CUMS significantly suppressed body weight and induced depressive-like behaviors characterized by prolonged immobility and reduced exploratory activity (P < 0.05). RES administration dose-dependently mitigated these behavioral impairments. Histological analyses showed that RES alleviated neuronal damage, inhibited microglial activation, decreased the levels of pro-inflammatory cytokines IL-1β and TNF-α, and increased anti-inflammatory IL-10 levels (P < 0.05). Targeted metabolomics revealed that RES restored hypothalamic neurotransmitter balance by elevating serotonin (5-HT), acetylcholine (ACh), and tryptophan (Trp) levels, while reducing norepinephrine (NE) and glutamate (Glu) concentrations (P < 0.05). Network pharmacology identified 85 putative antidepressant targets of RES, and integration with 700 hypothalamic differentially expressed genes (DEGs) highlighted significant enrichment in NF-κB, MAPK, and PI3K-Akt-mTOR signaling pathways. Western blotting confirmed RES inhibited TLR4/p38 MAPK/NF-κB activation and enhanced phosphorylation of PI3K/Akt/mTOR components (P < 0.05). These findings demonstrate that RES attenuates CUMS induced depressive-like behaviors in layer chicks primarily by modulating neuroinflammation and neurotransmitter metabolism via the MAPK and PI3K-Akt-mTOR pathways, offering mechanistic insights and supporting RES as a nutritional intervention to improve stress resilience in poultry.

Indexed as

Antidepressive AgentsChickensResveratrolStress, PhysiologicalStress, PsychologicalTranscriptomeAnimalsFemaleMetabolomicsNetwork PharmacologyAntidepressive AgentsResveratrolChronic stressDepressive-like behaviorNetwork pharmacologyPI3K-Akt-mTOR signaling pathwayResveratrol

Identifiers

PMID41795275
PMCPMC12989958

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.