Evidence map›Paper›PMID 41794711›Full record

ArticleBMC veterinary research2026

Development of an automated chemiluminescence immunoassay for detection of IgA antibodies against porcine epidemic diarrhea virus spike.

Duan Li, Daoping Zeng, Qi Wang, Leyi Zhang, Yanling Liu, Cuiqin Huang, Changxu Song, Lei Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Duan LiCollege of Life Sciences, Engineering Research Center for the Prevention and Control of Animal Original Zoonoiss, Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, 364012, P. R. China.
Daoping ZengYunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Wens Foodstuff Group Co., Ltd, Yunfu, Guangdong, 527400, P. R. China.
Qi WangCollege of Animal Science, State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, P. R. China.
Leyi ZhangCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611120, China.
Yanling LiuCollege of Animal Science, State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, P. R. China.
Cuiqin HuangCollege of Life Sciences, Engineering Research Center for the Prevention and Control of Animal Original Zoonoiss, Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, 364012, P. R. China. cuiqinh@126.com.
Changxu SongCollege of Animal Science, State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, P. R. China. cxsong2004@163.com.
Lei WangCollege of Life Sciences, Engineering Research Center for the Prevention and Control of Animal Original Zoonoiss, Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, 364012, P. R. China. wanglei3@lyun.edu.cn.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2023A1515010234National Key Research and Development Program of China 2021YFD2001205Natural Science Foundation of Fujian Province 2025J011026
6 · The paper itself

Abstract

backgroundPorcine epidemic diarrhoea virus (PEDV) causes substantial financial losses on pig farming worldwide. Monitoring PEDV-specific IgA antibodies is essential for assessing vaccine efficacy. In this study, we developed a fully automated chemiluminescence immunoassay (CLIA) for the detection of PEDV IgA antibodies in both serum and milk.

resultsThis S1-CLIA utilizes recombinant S1 protein-coated magnetic particles (S1-MPs) for antibody capture and an acridinium ester-labeled mouse anti-pig IgA monoclonal antibody (mpIgA-AE) to generate chemiluminescence signals. Under optimized conditions, S1-CLIA achieved a diagnostic sensitivity of 96.30% and specificity of 98.48%, with a cut-off index of 1.0. S1-CLIA exhibited excellent repeatability (coefficient of variation < 10%) and no cross-reactivity with sera positive for other common swine pathogens. Compared to a commercial enzyme-linked immunosorbent assay (ELISA), S1-CLIA achieved 93.93% agreement rate, but offered a significantly broader dynamic range (35.48-fold). Notably, S1-CLIA results correlated well with virus neutralization titers (VNT), yielding R² values of 0.6660 (serum) and 0.7639 (milk).

conclusionsWith capability of fully automated testing, this novel S1-CLIA presents a robust platform for large-scale evaluation of PEDV immunity.

Indexed as

Antibodies, ViralCoronavirus InfectionsImmunoglobulin ALuminescent MeasurementsPorcine epidemic diarrhea virusSpike Glycoprotein, CoronavirusSwine DiseasesAnimalsImmunoassayMilkSensitivity and SpecificitySwineAntibodies, ViralImmunoglobulin ASpike Glycoprotein, CoronavirusAntibody detectionChemiluminescent immunoassayIgAPorcine epidemic diarrhoea virusSpike

Identifiers

PMID41794711
PMCPMC13081319

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Registered trials

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