ArticleDiagnostic microbiology and infectious disease2026
Comparative evaluation of conventional and nanopore approaches in onychomycosis diagnosis.
Article in Diagnostic microbiology and infectious disease, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
backgroundOnychomycosis is a frequent and chronic nail infection caused by dermatophytes, yeast, and non-dermatophyte molds (NDM). Detection of the causative agent is relevant to overcoming recurrent infections and improving treatment. Combined microscopy and culture are the diagnostic gold standard; however, more sensitive tests are needed to speed detection. This work aimed to assess fungal nail diagnosis using conventional and molecular techniques. We explored the potential of nanopore sequencing for fungal detection.
methodsA total of 104 nail samples from 51 patients with onychomycosis and 53 individuals with healthy nails were subjected to potassium hydroxide(KOH) microscopy, culture, PCR, and nanopore sequencing. Sensitivity was calculated using a composite reference standard based on microscopy and culture.
resultsDistal subungual onychomycosis was the most frequent clinical presentation (43.1%). Infections were primarily caused by dermatophytes 60% (21), followed by yeast 22.9% (8) and NDM 11.4% (4), 5.7% (2) were mixed infections. The prevalent pathogens were T. rubrum (40%), T. mentagrophytes (14.3%), and C. albicans (14.3%). 90.2% (46/51) of patients were positive by KOH, 68.6% (35/51) by culture (58.8%, 30/51 positive in both), and 58.8%(30/51) were sequenced by nanopore. Fungal DNA sequences were identified in 65.7% (23/35) of culture-positive patients. Eight samples from healthy nails were positive for fungal DNA and were considered transient microbiota.
conclusionDermatophytes are the predominant causative agent; however, at least 30% of nail infections are caused by yeast and NDM. Conventional diagnosis remains challenging; alternatively, nanopore sequencing represents a complementary diagnostic tool that should be further optimized for clinical application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.