Evidence map›Paper›PMID 41793693›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2026

Prognostic Significance of miR-145, NANOG, OCT4, and KLF4 in Triple-Negative Breast Cancer.

Reham Sameh, Samia Hussein, Asmaa Atef Ibrahim, Ahmad Barakat Waley, Sara Mohammed Ibrahim, Aya M Bedawy, Mohammed Siam, Heba M Abdelgeleel

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Article in Asian Pacific journal of cancer prevention : APJCP, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Reham SamehDepartment of Pathology, Faculty of Medicine, Zagazig University, Egypt.
Samia HusseinMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt.
Asmaa Atef IbrahimMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt.
Ahmad Barakat WaleyMedical Oncology Department, Faculty of Medicine, Zagazig University, Egypt.
Sara Mohammed IbrahimClinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt.
Aya M BedawyMedical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Egypt.
Mohammed SiamGeneral Surgery Department, Faculty of Medicine, Suez University, Egypt.
Heba M AbdelgeleelDepartment of Pathology, Faculty of Medicine, Zagazig University, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAmong women, breast cancer is the leading cause of cancer-related deaths. OCT4, NANOG, and KLF4 are markers of cancer stem cells. miR-145 is a tumor suppressor in many cancers. We aimed to estimate the expression of miR-145 and cancer stem cell markers in triple-negative breast cancer (TNBC) and to investigate their correlations with clinicopathological characteristics and outcomes in these patients.

methodsThe study included 90 female patients with TNBC. Grade III represented 64.4% of cases, while Grades I and II represented 11.1% and 24.4%, respectively. Seventy-two patients (80%) had lymph node involvement. Breast tissues from malignant and adjacent control tissues were used for RNA extraction and subsequent analysis of miR-145 expression. Histopathological and immunohistochemical analyses were also performed.

resultsIt was found that lymph node (LN)-positive tumors exhibited higher OCT4 levels compared to LN-negative tumors (P = 0.039). Additionally, tumors with extensive intraductal invasion and relapse showed lower KLF4 levels (P = 0.02 and <0.001, respectively). Downregulated miR-145 expression was associated with higher stages, relapse, and mortality (P < 0.001 for each), LN involvement (P = 0.002), and capsular invasion (P = 0.02). There were significant strong positive correlations between miR-145 and DFS (r = 0.920, P < 0.001) and OS (r = 0.813, P < 0.001). However, significant weak negative correlations were found with KLF4 (r = -0.242, P = 0.022), NANOG (r = -0.305, P = 0.009), OCT4 (r = -0.255, P = 0.014), tumor size (r = -0.247, P = 0.019), and the number of positive LNs (r = -0.481, P < 0.001). OCT4 levels showed significant positive correlations with NANOG (r = 0.328, P = 0.002) and KLF4 expression (r = 0.344, P = 0.001). Moreover, a significant positive correlation was detected between KLF4 levels and DFS (r = 0.255, P = 0.015). The log-rank test showed a significant association of KLF4 with DFS (P = 0.005).

conclusionmiR-145 and KLF4 are possible prognostic markers in TNBC. This was reflected by the positive correlations between miR-145 and both DFS and OS, and between KLF4 levels and DFS.

Indexed as

Biomarkers, TumorKruppel-Like Transcription FactorsMicroRNAsNanog Homeobox ProteinNeoplasm Recurrence, LocalOctamer Transcription Factor-3Triple Negative Breast NeoplasmsAdultCase-Control StudiesFemaleFollow-Up StudiesGene Expression Regulation, NeoplasticHumansKruppel-Like Factor 4Lymphatic MetastasisMiddle AgedBiomarkers, TumorKLF4 protein, humanKruppel-Like Factor 4Kruppel-Like Transcription FactorsMicroRNAsMIRN145 microRNA, humanNanog Homeobox ProteinNANOG protein, humanOctamer Transcription Factor-3POU5F1 protein, humanklf4miR-145NanogOct4Triple-negative breast cancer

Identifiers

PMID41793693
PMCPMC13576480

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