Evidence map›Paper›PMID 41793511›Full record

ArticlePlant cell reports2026

Overexpression of GhAAP3 increases Helicoverpa armigera feeding by altering hormonal and metabolic composition in cotton.

Amjad Hussain, Hakim Manghwar, Muna Alariqi, Lu Yu, Guangqin Yang, Ying Wang, Xiufeng Han, Qurban Ali, Zhongping Xu, Shuangxia Jin

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Amjad HussainHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Hakim ManghwarLushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang, 332900, China.
Muna AlariqiHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Lu YuHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Guangqin YangHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Ying WangHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Xiufeng HanHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Qurban AliDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, Abu-Dhabi, United Arab Emirates.
Zhongping XuHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. xzp@mail.hzau.edu.cn.
Shuangxia JinHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. jsx@mail.hzau.edu.cn.

Funding

Agriculture Science and Technology Major Project, and the Development Fund for Xinjiang Talents XL XL20240
6 · The paper itself

Abstract

key messageGhAAP3 negatively regulates cotton resistance to Helicoverpa armigera by downregulating key defense pathways, impairing the plant's chemical defense, and enhancing nutritional suitability for herbivorous pests. Cotton (Gossypium spp.), a fundamental global fiber crop, is highly susceptible to insect pests, including Helicoverpa armigera. Amino acid/auxin permeases (AAAPs) are crucial membrane transporters involved in plant development and stress responses. In this study, we overexpressed amino acid permease 3 (GhAAP3) in upland cotton to evaluate its function in defense against H. armigera. The larvae fed on GhAAP3-overexpressing plants (AP3-1 and AP3-2) exhibited higher growth and feeding by 95.85% and 220.83%, respectively, compared to larvae fed on wild-type (WT) plants. Transcriptomic analysis revealed global downregulation of key defense pathways, including jasmonic acid (JA) biosynthesis and signaling, mitogen-activated protein kinase (MAPK) signaling, and phenylpropanoid and terpenoid biosynthesis pathways, in AP3. Consistently, GhAAP3 overexpression caused a marked reduction in JA-related hormones (JA, methyl jasmonate (MeJA), and jasmonic acid-isoleucine (JA-Ile)) by 45.75-87.41% and key defensive secondary metabolites (flavonoids and gossypol) by 26.35-56.38%. Conversely, free amino acids and soluble sugar contents increased significantly in AP3-overexpressing transgenic plants. Our findings demonstrate that GhAAP3 negatively regulates cotton resistance against H. armigera by downregulating key defense-related phytohormones and secondary metabolites, while concurrently elevating amino acid and sugar levels. This together impairs the plant's chemical defense and enhances its nutritional suitability for herbivorous pests.

Indexed as

GossypiumHelicoverpa armigeraPlant Growth RegulatorsPlant ProteinsAcetatesAnimalsCyclopentanesGene Expression Regulation, PlantHerbivoryLarvaOxylipinsPlants, Genetically ModifiedAcetatesCyclopentanesjasmonic acidmethyl jasmonateOxylipinsPlant Growth RegulatorsPlant ProteinsCottonGhAAP3Helicoverpa armigeraInsect herbivoresPlant defense

Identifiers

PMID41793511

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.