ArticleFish physiology and biochemistry2026
Chronic Thermal Stress During Early Zebrafish (Danio rerio) Development Induces Morphological, Molecular, and Liver Histopathological Changes.
Article in Fish physiology and biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Combined Transcriptomic and Histological Profiling Uncover Hepatic Regulatory Hierarchy of TriploidBiology · 2026Article
- Chronic thermal stress affects growth, liver morphology and molecular responses but allows recovery at optimal temperature in juvenile zebrafish (Danio rerio).Journal of fish biology · 2026Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Temperature is a critical abiotic factor mediating the physiological fitness of fish. While the impact of acute high-temperature exposure is well documented in teleost fishes, the effects of chronic thermal stress, especially during early stages, remain poorly understood. This study examined the effects of prolonged exposure to elevated temperatures (34 ºC) on zebrafish (Danio rerio) development, survival, molecular responses and liver histology during pre-independent (24-120 h post fertilisation [hpf]) and independently feeding (240-480 hpf) stages. While survival was not affected by elevated temperature, normal development was significantly impaired in both stages. Compared to control conditions (28 °C), heat exposure (34 ºC) increased the incidence of deformities, including spinal and yolk sac abnormalities during the pre-independent feeding stage, and spinal and growth-related deformities during independent feeding. Heat-induced changes in gene expression were most evident during independent feeding, with the upregulation of heat shock proteins (HSP90AA1, SERPINH1A, SERPINH1B) and downregulation of growth (GH, GHRA, IGF-1) genes. By 480 hpf, pronounced liver changes were also observed in heat-exposed fish, characterised by marked cellular vacuolation and hepatic glycogen accumulation. These results highlight the complex, age-specific responses to chronic thermal stress, reflected in altered heat shock response, development, and hepatocyte morphology. These findings contribute to the assessment of stage-specific responses relevant to biomarker development for prolonged heat exposure in developing finfish.
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Registered trials
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