Evidence map›Paper›PMID 41793249›Full record

ArticleJournal of experimental botany2026

Simultaneous overexpression of three enzymes of chloroplast metabolism fails to improve CO2 assimilation or biomass.

Pauline Lemonnier, Shellie Wall, Hussein Gherli, Beatriz Moreno-Garcia, Chidi Afamefule, Tracy Lawson, Christine A Raines, Patricia E Lopez-Calcagno

Abstract read
In one paragraph

Article in Journal of experimental botany, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pauline LemonnierSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0001-9443-4392
Shellie WallSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0001-7238-7679
Hussein GherliSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0002-9714-4179
Beatriz Moreno-GarciaSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0002-3485-1781
Chidi AfamefuleSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0002-6412-7903
Tracy LawsonSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0002-4073-7221
Christine A RainesSchool of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.ORCID 0000-0001-7997-7823
Patricia E Lopez-CalcagnoSchool of Natural and Environmental Sciences, Newcastle University, Newcastle NE1 7RU, UK.ORCID 0000-0003-2436-8988

Funding

BBSRC BB/J004138/1Bill and Melinda Gates Foundation
6 · The paper itself

Abstract

Ensuring an adequate food supply amidst a growing global population and climate change challenges necessitates innovative strategies to enhance crop productivity. Previous studies have demonstrated that the simultaneous stimulation of different photosynthesis-related processes can increase the rate of photosynthetic carbon assimilation and plant biomass. This study evaluates an approach based on modelling aimed at simultaneously increasing photosynthetic and sink capacities in Nicotiana tabacum by overexpressing three key enzymes: sedoheptulose-1,7-bisphosphatase (SBPase), fructose-1,6-bisphosphate aldolase (FBP aldolase), and ADP-glucose pyrophosphorylase (AGPase). Our results showed that this strategy does not significantly improve growth or carbon assimilation in Nicotiana tabacum under the tested conditions. This suggests that while the model informing our work offers a valuable framework, its application may require adjustments based on species and environmental conditions. Future research should explore these genetic modifications in species with larger sink capacities and under a range of growth conditions to fully realize the potential of photosynthetic optimization.

Indexed as

Carbon DioxideChloroplastsFructose-Bisphosphate AldolaseGlucose-1-Phosphate AdenylyltransferaseNicotianaPhosphoric Monoester HydrolasesPlant ProteinsBiomassPhotosynthesisPlants, Genetically ModifiedCarbon DioxideFructose-Bisphosphate AldolaseGlucose-1-Phosphate AdenylyltransferasePhosphoric Monoester HydrolasesPlant Proteinssedoheptulose-bisphosphataseADP-glucose pyrophosphorylasecarbon assimilationcrop productivityfructose-1,6-bisphosphate aldolasemodel-informed genetic manipulationNicotiana tabacumphotosynthesis optimizationsedoheptulose-1,7-bisphosphatasesink capacityyield improvement

Identifiers

PMID41793249
PMCPMC13293071

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.