ArticleBMC veterinary research2026
Evaluation of recombinant Taenia multiceps HSP70 antigen in a dipstick-based rapid diagnostic test for ovine coenurosis.
Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundCoenurus cerebralis, the larval stage of Taenia multiceps, causes coenurosis, a severe neurological disease in sheep associated with significant economic losses. Ante-mortem diagnosis remains difficult due to non-specific clinical signs and the limited applicability of laboratory-based methods under field conditions. Heat shock protein 70 (HSP70) has previously shown promising immunoreactivity in laboratory assays, suggesting its potential as a diagnostic antigen. This study aimed to evaluate the format-dependent performance of a laboratory-validated recombinant antigen when translated into a dipstick-based rapid diagnostic assay for ovine coenurosis.
resultsDespite strong immunoreactivity in Western blot analysis, rTmHSP70 failed to generate a visible test line signal in the dipstick assay under all tested conditions. In contrast, a partially purified hydatid cyst antigen produced visible signals but also caused non-specific reactions and false-positive results. These findings demonstrate that rTmHSP70, although suitable for laboratory-based assays, is not compatible with dipstick-format rapid diagnostics.
conclusionThe study underscores the importance of assay format compatibility, antigen purity, and epitope accessibility in the development of reliable field-applicable diagnostic tests for coenurosis. The findings demonstrate that strong immunoreactivity in laboratory-based assays does not necessarily translate into functionality in rapid diagnostic formats, highlighting critical format-dependent limitations in assay development.
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