Evidence map›Paper›PMID 41792459›Full record

ArticleScientific reports2026

Isolation of aerobic denitrifying bacteria Stutzerimonas stutzeri and its application in coking wastewater treatment.

Khoula Naseer, Kainat Ashfaq, Ammara Shamim, Maryam Saeed, Usaal Tahir, Alia Batool, Qurban Ali, Daoud Ali

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Khoula NaseerKey Laboratory of Resources and Environmental Systems Optimization, Ministry of Education, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, China. naseerkhoula@gmail.com.
Kainat AshfaqDepartment of Food Sciences, University of the Punjab, Lahore, Pakistan.
Ammara ShamimDepartment of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Maryam SaeedDepartment of Food Sciences, University of the Punjab, Lahore, Pakistan.
Usaal TahirInstitute of molecular biology and biotechnology, The university of Lahore, Lahore, Pakistan.
Alia BatoolDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Qurban AliDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences, University of the Punjab, Lahore, Pakistan. saim1692@gmail.com.
Daoud AliDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Improvements in denitrifying bacteria since 1970 have optimized the activity of autotrophic denitrification and heterotrophic nitrification. Synchronous autotrophic biofilms, denitrification, and nitrification have enhanced nitrogen removal from industrial wastewater, but so far, this process relies on variables such as dissolved oxygen, pH, and carbon sources. The aerobic denitrification capability of the coking wastewater-derived Stutzerimonas stutzeri KA1 is quantified in the present investigation to enhance nitrogen removal potential under various environmental conditions. The strain was studied for its ability to isolate nitrate using sodium acetate as the carbon source, and the effectiveness of the microorganism was tested at different dissolved oxygen, pH, and C/N levels. Findings indicate that strain KA1 achieved nearly 100% nitrate elimination within 40 h at pH 6-10 and an optimal C/N ratio of 8, demonstrating resilience under both aerobic and anaerobic conditions. Strain KA1 showed robust denitrification across a wide range of dissolved oxygen levels (0-100%), including stable nitrate reduction under aerobic bulk conditions. Bioaugmentation experiments conducted in a Sequential Batch Reactor (SBR) confirmed that strain KA1 significantly enhanced nitrogen removal, particularly under saline wastewater conditions, exceeding the control systems. In conjunction with nitrogen elimination, the strain also demonstrates robust Chemical Oxygen Demand (COD) reduction, efficiently degrading organic pollutants in coking wastewater. Compared to other studies, Stutzerimonas stutzeri KA1 demonstrated higher denitrification efficiency and greater resistance to environmental stress, making it a scalable, cost-effective solution for denitrifying industrial wastewater. The results offer valuable insights into optimizing wastewater treatment parameters and can advance microbial biotechnology to foster sustainable environmental practices.

Indexed as

CokeDenitrificationWastewaterWater PurificationAerobiosisBioreactorsHydrogen-Ion ConcentrationNitratesNitrogenCokeNitratesNitrogenWastewaterAerobic denitrificationBioaugmentationCOD reductionCoking wastewaterNitrate removalSequential Batch Reactors (SBR)Stutzerimonas stutzeri KA1

Identifiers

PMID41792459
PMCPMC12979746

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