Evidence map›Paper›PMID 41792256›Full record

ArticleApplied microbiology and biotechnology2026

Microbial-induced calcite precipitation by indigenous alkaliphilic bacteria: a dual-enzyme strategy for crack-healing in cementitious materials.

Mohammad Shiri, Abbas Bahari, Mohammad Mahdi Khani Sarbangholi, Nikolai IvanovichVatin, Hamid Rahmani, Amin Ebrahimpour Bozorg, Elahe Gholamian, Mobina Kordlou

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In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohammad ShiriDepartment of Civil Engineering, University of Zanjan, Zanjan, Iran.
Abbas BahariResearch Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran. Bahari@znu.ac.ir.
Mohammad Mahdi Khani SarbangholiDepartment of Civil Engineering, University of Zanjan, Zanjan, Iran.
Nikolai IvanovichVatinAdvanced Engineering School for Digital Engineering, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia.
Hamid RahmaniDepartment of Civil Engineering, University of Zanjan, Zanjan, Iran.
Amin Ebrahimpour BozorgDepartment of Civil Engineering, University of Zanjan, Zanjan, Iran.
Elahe GholamianResearch Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran.
Mobina KordlouResearch Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial-induced calcite precipitation (MICP) offers a sustainable strategy for extending the service life of concrete through autonomous crack healing, yet the high alkalinity of cementitious environments restricts microbial viability. In this study, more than 200 indigenous bacterial isolates collected from extreme environments across Iran were systematically screened for urease and carbonic anhydrase (CA) activities. A dual-enzyme activity index (EAI) was developed to quantitatively rank their calcification potential. Four robust spore-forming strains-Bacillus subtilis, Sporosarcina pasteurii, Bacillus sphaericus, and the environmental isolate E10.2-were identified as top candidates based on high EAI values, sporulation capacity, and survival at pH 13.5. These strains retained at least 70% of their enzymatic activity after alkaline exposure and precipitated up to 89% more CaCO

Indexed as

BacteriaCalcium CarbonateCarbonic AnhydrasesConstruction MaterialsUreaseBacillaceaeBacillusBacillus subtilisChemical PrecipitationHydrogen-Ion ConcentrationSporosarcinaCalcium CarbonateCarbonic AnhydrasesUreaseAlkaliphilic spore-forming bacteriaCarbonic anhydrase activityMICPNative microbial isolatesSelf-healing concreteUrease activity

Identifiers

PMID41792256
PMCPMC12967677

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.