Evidence map›Paper›PMID 41791384›Full record

ArticleStem cell reports2026

Establishing mouse forebrain organoids as models of intrinsic cortical network assembly.

Sebastian Hernandez, Hunter E Schweiger, Isabel Cline, Gregory A Kaurala, Ash Robbins, Daniel Solis, Samira Vera-Choqqueccota, Jinghui Geng, Tjitse van der Molen, Francisco Reyes and 10 more

Abstract read
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Sebastian HernandezGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Hunter E SchweigerGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Isabel ClineGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Gregory A KauralaGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Ash RobbinsGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Daniel SolisGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Samira Vera-ChoqqueccotaGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Jinghui GengGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Tjitse van der MolenGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA; Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Francisco ReyesGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Biotechnology Program, Berkeley City College, Berkeley, CA 94704, USA.
Chinweike Norman AsogwaDepartment of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Kateryna VoitiukGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Mattia ChiniInstitute of Developmental Neurophysiology, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Marco RolandiGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Sofie R SalamaGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Bradley M ColquittGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Molecular, Cellular and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Tal SharfGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
David HausslerGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Mircea TeodorescuGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA. Electronic address: mteodore@ucsc.edu.
Mohammed A Mostajo-RadjiGenomics Institute, University of California, Santa Cruz, Santa Cruz, CA 95064, USA. Electronic address: mmostajo@ucsc.edu.

Funding

Nanoparticle Tracking Analyzer (NTA) for the Center for Live Cell GenomicsRM1HG011543 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI HAUSSLER, DAVID H, SALAMA, SOFIE REDA · 2021 to 2025
$12.0M
Data Resource and Administrative Coordination Center for the Scalable and Systematic Neurobiology of Psychiatric and Neurodevelopmental Disorder Risk Genes ConsortiumU24MH132628 · NIMH · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI DAVID H HAUSSLER, Tomasz Nowakowski · 2023 to 2026
$7.9M
Human Brain Single-Cell Genomics ExplorerU24NS146314 · NINDS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI HAUSSLER, DAVID H, ZHU, JINGCHUN · 2025 to 2025
$3.7M
Dissecting the role of NOTCH2NL genes in human brain development and neurological disorders associated with chromosome 1q21.1 distal duplications and deletions.R01MH120295 · NIMH · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI SALAMA, SOFIE REDA · 2020 to 2024
$3.7M
Molecular mechanisms of motor skill stabilizationR01NS138781 · NINDS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Bradley Mark Colquitt · 2024 to 2026
$1.4M
NHGRI NIH HHS RM1 HG011543NIMH NIH HHS R01 MH120295NIMH NIH HHS U24 MH132628NINDS NIH HHS R01 NS138781NINDS NIH HHS U24 NS146314
6 · The paper itself

Abstract

The mouse cortex is a canonical model for studying how functional neural networks emerge, yet it remains unclear which topological features arise from intrinsic cellular organization versus sensory input. Mouse forebrain organoids provide a powerful system to investigate these intrinsic mechanisms. We generated dorsal (DF) and ventral (VF) forebrain organoids from mouse pluripotent stem cells and tracked their development using longitudinal electrophysiology. DF organoids showed progressively stronger network-wide correlations, while VF organoids developed more refined activity patterns with enhanced small-world topology and increased modular organization. Both organoid types form small-world networks, but their topological organization differs. These differences emerge without extrinsic inputs and correlate with Pvalb

Indexed as

Cerebral CortexNerve NetOrganoidsProsencephalonAnimalsInterneuronsMiceNeurodevelopmentPluripotent Stem Cellsbrain organoidscortical developmentmouse organoidsmultielectrode arraysparvalbumin

Identifiers

PMID41791384
PMCPMC13083800

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.