Protocol to reconstitute motor protein clustering on vesicle membranes using a DNA scaffold-based system in vitro.

Rui Jiang, William O Hancock

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Rui JiangIntercollege Program in Integrative and Biomedical Physiology, Pennsylvania State University, University Park, PA 16802, USA; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: jiangr@berkeley.edu.

William O HancockIntercollege Program in Integrative and Biomedical Physiology, Pennsylvania State University, University Park, PA 16802, USA; Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA; Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA.

Funding

Research Training in Physiological Adaptations to StressT32GM108563 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI CANTORNA, MARGHERITA T, KORZICK, DONNA HOPE · 2014 to 2023

$2.2M

NIGMS NIH HHS T32 GM108563

6 · The paper itself

Abstract

Cytoskeletal motor proteins drive long-range intracellular transport, but how their organization on vesicle membranes affects motility remains poorly understood. Here, we present a protocol to reconstitute kinesin-1 clustering on synthetic liposomes using a DNA-based scaffold. We describe steps for functionalizing liposomes with kinesin-1, inducing DNA-mediated motor clusters, and analyzing liposome motility on surface-immobilized microtubules. This protocol enables quantitative investigation of motor organization in vesicle transport and is adaptable to other cytoskeletal motors. For complete details on the use and execution of this protocol, please refer to Jiang et al.

Indexed as

DNAKinesinsLiposomesMolecular Motor ProteinsMicrotubulesDNAKinesinsLiposomesMolecular Motor ProteinsBiophysicsMicroscopySingle-molecule Assays

Identifiers

PMID41790551

DOI10.1016/j.xpro.2026.104409

PMCPMC12992951

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.