Evidence map›Paper›PMID 41790321›Full record

ArticleFolia microbiologica2026

Combined application of Vancomycin and Levofloxacin potentiates the antimicrobial activity against MRSA: a response surface methodology-based study.

Anuka Sahoo, Alakesh Maity, Biswamitra Das, Ratish Chandra Paul, Payel Paul, Poulomi Chakraborty, Bhaskar Narayan Chaudhuri, Mahashweta Mitra Ghosh, Sharmistha Das, Prosun Tribedi

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In one paragraph

Article in Folia microbiologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Anuka SahooMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India.
Alakesh MaityMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India.
Biswamitra DasMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India.
Ratish Chandra PaulSusrut Eye Foundation & Research Centre, HB 36/A/1, Near Water Tank No 12, Salt Lake City Sector 3, West Bengal, 700106, India.
Payel PaulMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India.
Poulomi ChakrabortyMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India.
Bhaskar Narayan ChaudhuriDepartment of Microbiology & Molecular Biology, Peerless Hospitex Hospital & Research Center Ltd., 360, Panchasayar, Kolkata, West Bengal, 700094, India.
Mahashweta Mitra GhoshPostgraduate and Research Department of Microbiology, St. Xavier's College (Autonomous), Kolkata, West Bengal, 700016, India.
Sharmistha DasMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. sharmisthad986@gmail.com.ORCID http://orcid.org/0000-0001-7752-8549
Prosun TribediMicrobial Ecology Research Laboratory, Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India. tribedi.prosun@gmail.com.ORCID http://orcid.org/0000-0002-6567-006X

Funding

The Neotia University, India TNU/R&D/MG/24/02The Neotia University, India TNU/R&D/MP/2021/010
6 · The paper itself

Abstract

Antimicrobial resistance (AMR) ranks among the top 10 global health threats, severely limiting the effectiveness of available therapeutics. methicillin resistant Staphylococcus aureus (MRSA) poses a major concern due to its multidrug resistance and restricted treatment options. The decline in the discovery of new antibiotics has necessitated alternative strategies such as combinatorial applications. The present study described the combined potential of vancomycin and levofloxacin against 10 MRSA strains. Checkerboard assays demonstrated 2 to sixfold and 3 to fivefold reductions in minimum inhibitory concentration (MIC) of vancomycin and levofloxacin, respectively, when used in combination, confirming enhanced antimicrobial potential. Fractional Inhibitory Concentration Index (FICI) also suggested prominent interactions between the selected antibiotics against MRSA when applied together. To further optimize the doses of the combination of the selected antibiotics against MRSA, Response Surface Methodology (RSM) was explored. The RSM models exhibited strong reliability (R2 = 0.9381–0.9903) and non-significant lack of fit (p > 0.05), validating models’ robustness. The optimized combinations were further validated in-vitro through microbial growth profile, suggesting that the actual observations closely aligned with the predicted microbial growth inhibition. Collectively, RSM-optimized doses of vancomycin-levofloxacin could demonstrate enhanced antibacterial potential in combination, offering a rational framework for precision-based therapy against MRSA-borne infections.

Indexed as

Combinatorial therapyLevofloxacinMethicillin resistant Staphylococcus aureusResponse surface methodologyVancomycin

Identifiers

PMID41790321

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.