ArticleJournal of ginseng research2026
Development of a MIRA-CRISPR/Cas12a-based nucleic acid detection system for the discrimination of
Article in Journal of ginseng research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Rapid On-Site Detection ofFoods (Basel, Switzerland) · 2026Article
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8 authors.
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Abstract
Background: Accurate identification of closely related herbal species is essential for ensuring the safety, efficacy, and authenticity of traditional Chinese medicine (TCM) products. Methods: We developed a nucleic acid detection platform that integrates multienzyme isothermal rapid amplification (MIRA) with CRISPR/Cas12a-based fluorescence or lateral flow assay (LFA) for the rapid discrimination of PG and PQ. By targeting highly divergent mitochondrial gene regions, we designed species-specific crRNAs that enabled precise identification of PG and PQ with high sensitivity and specificity. Results: By optimizing the conditions, the system can effectively distinguish PG and PQ, with a fluorescence detection limit of 10 Conclusion: This method offers a promising solution for point-of-care testing (POCT) in TCM quality control and provides a foundation for broader applications of CRISPR-based molecular authentication in herbal medicine.
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