ArticleAlpha psychiatry2026
UPLC-MS/MS Analytical Method for the Simultaneous Quantification of Diazepam, Nordazepam, and Oxazepam in Patients With Alcohol Dependence.
Article in Alpha psychiatry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: To establish a method for the simultaneous quantification of diazepam (DIA) and its active metabolites, nordazepam (NorD) and oxazepam (OXAZ), and provide a reference range for therapeutic concentrations in patients with alcohol dependence. Methods: Simple and direct protein precipitation was used to extract the biological samples. Subsequent separation was performed on an Agilent XDB-C18 column (50 mm × 4.6 mm, 1.8 μm) with a column temperature maintained at 35 °C and a flow rate of 0.5 mL/min via ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The mobile phase consisted of methanol-water containing 5 mM ammonium formate (75:25, v/v). Detection was conducted using electrospray ionization in multiple reaction monitoring modes: Results: The key parameters of the bioanalytical method were validated: the average extraction recovery was 95%-101% (CV <6%); calibration curves exhibited good linearity over the concentration range (R Conclusions: We developed and validated a rapid, simple, and economic UPLC-MS/MS method for the quantification of DIA, NorD, and OXAZ in human serum. The method is well-suited for the determination of serum levels of DIA and its active metabolites in patients with alcohol dependence, and could be further applied to TDM and subsequent studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.