Evaluation of the iFalcon

Accurate intraocular pressure (IOP) measurement is fundamental for both clinical ophthalmology and experimental research. Study evaluated the performance of iFalconTMV100 rebound tonometer in four enucleated rabbit eyes. We employed a stepwise pressure-increasing protocol to generate a physiologically relevant IOP range (5–55 mmHg). At each target pressure, measurements were taken concurrently with the tonometer (using the rabbit-specific mode) and the reference sensor. We used linear regression and Bland-Altman analysis to assess agreement across the entire dataset, supplemented by a segmented analysis of normal and elevated IOP ranges. Overall linear regression revealed a strong correlation between the iFalconTMV100 Vet and the reference sensor(R² = 0.9712, p < 0.001); however, interval-dependent bias was present. The positive intercept (0.8951) suggested a tendency for the tonometer to overestimate IOP at low measurement range, while slope < 1 suggested systematic underestimation at higher pressures. Bland-Altman analysis further confirmed this systematic underestimation bias at central corneal position. The iFalconTMV100 rebound tonometer showed strong agreement with direct sensor measurements across a broad pressure range (5–55 mmHg) in an ex vivo rabbit model, supporting its potential utility for IOP assessment in this setting, with limitations noted in extreme pressure ranges.