Evidence map›Paper›PMID 41784871›Full record

ArticleTropical animal health and production2026

Molecular detection of Mollicutes agents in reproductive system of cattle: a three-year study in Rio Grande do Sul, southern Brazil (2022-2024).

Helena Castro Alves Wessely, Evelyn Kaus Dotto, Alice Sampaio Moraes da Costa, Fernanda Silveira Flôres Vogel, Juliana Felipetto Cargnelutti, Alfredo Quites Antoniazzi

Abstract read
In one paragraph

Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

6 authors.

Helena Castro Alves WesselyCentro de Ciências Rurais, Programa de Pós-Graduação de Medicina Veterinária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil.ORCID http://orcid.org/0009-0000-2596-2968
Evelyn Kaus DottoCentro de Ciências Rurais, Programa de Pós-Graduação de Medicina Veterinária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil.ORCID http://orcid.org/0000-0002-7004-555X
Alice Sampaio Moraes da CostaCentro de Ciências Rurais, Graduação em Medicina Veterinária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil.ORCID http://orcid.org/0009-0003-5199-7963
Fernanda Silveira Flôres VogelCentro de Ciências Rurais, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil.ORCID http://orcid.org/0000-0003-1351-9912
Juliana Felipetto CargneluttiCentro de Ciências Rurais, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil. juliana.cargnelutti@ufsm.br.ORCID http://orcid.org/0000-0002-3160-3643
Alfredo Quites AntoniazziCentro de Ciências da Saúde, Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, CEP 7105900, Brasil. alfredo.antoniazzi@ufsm.br.ORCID http://orcid.org/0000-0003-4866-5944

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rio Grande do Sul state, in southern Brazil, is one of the country’s largest cattle producers but reports few cases of genital infections caused by Mollicutes, specially Ureaplasma diversum and Mycoplasma bovis. The clinical signs associated with these bacteria can be easily confused with those caused by bovine herpesvirus (Varicellovirus bovinealpha1), Campylobacter fetus, and Tritrichomonas fetus, which complicates diagnosis. This study aimed to assess the occurrence of Mollicutes in cattle from Rio Grande do Sul between April 2022 and November 2024. Vulvo-vaginal and preputial lavage samples submitted for routine diagnosis at a Bacteriology Laboratory, were tested using PCR. Samples were first tested for U. diversum and M. bovis. Those testing negative were further examined for Mycoplasma bovigenitalium and Mycoplasma spp., and if still negative, for the Mollicutes class to detect other species. In total, 136 samples from 16 beef cattle farms in central, southern, and western regions of the state were analyzed. These included herds with reproductive disorders (n = 11) and asymptomatic animals (n = 5). U. diversum was detected in 44 samples (32%) from nine herds, both with and without reproductive issues. M. bovis was identified in one sample (1%) from a herd with reproductive problems. M. bovigenitalium was found in 11 preputial samples (9%) from three affected herds. PCR also detected Mycoplasma spp. in 25 samples (18%) and Mollicutes class in 31 samples (23%). These results confirm the presence of Mollicutes in reprodutive system in both, symptomatic and asymptomatic animals, highlighting their circulation in herds from Southern Brazil.

Indexed as

Cattle DiseasesTenericutesAnimalsBrazilCattleFemalePolymerase Chain ReactionUreaplasmaCattleMollicutesMycoplasma spp.PCRUreaplasma diversumVulvovaginitis

Identifiers

PMID41784871
PMCPMC12963107

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.