Texture preference task: a rapid and training-independent behavioural assay to evaluate somatosensory perception in freely moving mice.

Somatosensory perception, mediated though the whiskers and paws, plays a critical role in how rodents explore and interact with their environment. Whereas strokes are associated with devastating motor deficits, they also frequently disrupt tactile processing, yet most commonly used behavioural assays emphasize gross motor coordination rather than spontaneously driven sensory behaviour. To address this gap, we developed a simple open-field texture preference assay for mice. The task leverages innate exploratory behaviour, requires no training, and provides a rapid automated measure of tactile preference before and after cortical stroke. Here, we offer a reproducible and accessible approach for assessing post-stroke somatosensory deficits. Essential highlights Provides a non-invasive and training-free assay of tactile preference in freely moving mice. Enables sensitive detection of post-stroke sensory impairments that complement standard motor tests. Adaptable for diverse experimental contexts, including recovery studies and therapeutic interventions. Overall, this protocol offers an efficient, low-cost addition to behavioural testing batteries in preclinical stroke research. Beyond stroke, the task may be broadly applicable to studies of tactile processing, sensory preference, and environmental enrichment in freely moving mice.