ArticleFrontiers in veterinary science2026
Evidence for modulation of the fecal microbiota profile by diet in lactating buffalo.
Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Effect of different dietary regimes on gut microbiota in water buffalo calves during the preweaning period.BMC microbiology · 2026Article
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8 authors.
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Abstract
Introduction: The gastrointestinal microbiota of ruminants plays a crucial role in health, influencing immune responses, nutrient metabolism, and environmental impact. While the ruminal microbiota has been widely investigated, the hindgut microbiota, particularly the fecal microbiota, remains less explored. Diet strongly shapes microbial communities, thereby affecting digestion, metabolic pathways, and methane emissions. Next-generation sequencing enables detailed microbiota profiling; however, no studies have characterized the fecal microbiota of Italian Mediterranean buffaloes in relation to diet. This study aimed to evaluate the bacterial composition and dietary influences on the fecal microbiota of dairy buffaloes. Methods: Over 6 months, monthly pooled fecal samples were collected from ~10 to 15% of lactating buffaloes across 10 farms. Concurrently, dietary data were recorded, and total mixed ration samples were analyzed for physicochemical properties and fatty acid profiles. DNA was extracted using the Quick-DNA™ kit, followed by 16S rRNA sequencing on an Illumina MiSeq System. Statistical analyses in R included alpha and beta diversity, differential abundance testing, and one-way ANOVA ( Results: In total, 10 phyla, 13 classes, 26 orders, 47 families, 86 genera, and 120 species were identified. Conclusion: This study provides the first characterization of the fecal microbiota of Italian Mediterranean dairy buffaloes and highlights its responsiveness to diet. Findings underscore the potential of fecal microbiota as a non-invasive biomarker for evaluating dietary effects, with implications for animal health, productivity, and environmental sustainability.
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