ArticleInfection and drug resistance2026
Clinical Utility of a Droplet Digital PCR-Based Assay for Quantitative Detection of
Article in Infection and drug resistance, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Purpose: Patients and Methods: An observational cohort of 193 patients with suspected fungal pneumonia was enrolled. The cell-free DNA (cfDNA) in bronchoalveolar lavage fluid (BALF) and serum samples was prepared and quantitated via a ddPCR assay targeting the mitochondrial large subunit rRNA gene of Results: The cases with complete data were classified into a PCP group (N=30) and a non-PCP group (N=139). This ddPCR assay demonstrated a sensitivity of 91.3% and a specificity of 96.8% for BALF, in contrast to a sensitivity of 57.1% and a specificity of 100% for serum. The area under the curve of 0.896 for diagnosis was obtained via ddPCR assay, compared with 0.627 via G-test ( Conclusion: The ddPCR assay exhibited robust diagnostic performance for PCP in BALF samples and cfDNA copies may serve as an indicator for improving the management of patients.
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