Evidence map›Paper›PMID 41782371›Full record

ArticlePlant communications2026

A phased small interfering RNA acts in an OsAGO2-dependent manner to mediate OsARF7 cleavage and promote male fertility in rice.

Liang Shao, Keli Qiu, Rui Xu, Jiankai Zhou, Junwen Yang, Jun Liu, Yunfei Yang, Lingling Li, Biao Xie, Yu Wang and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Liang ShaoNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Keli QiuNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Rui XuNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Jiankai ZhouNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Junwen YangNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Jun LiuNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Yunfei YangNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Lingling LiNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Biao XieNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Yu WangNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China; Anhui Province Key Laboratory of Rice Germplasm Innovation and Molecular Improvement, Anhui Academy of Agricultural Sciences, Hefei 230036, China.
Jiaojiao LiCAS Center for Excellence in Molecular Plant Sciences, School of Life Sciences, University of Science and Technology of China, Huangshan Road 443, Hefei 230027, China.
Xiaoshuang LiuCollege of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Meng YuanCenter for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Yachun YangAnhui Province Key Laboratory of Rice Germplasm Innovation and Molecular Improvement, Anhui Academy of Agricultural Sciences, Hefei 230036, China.
Yunfei LiNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Pengcheng WeiNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China; College of Agronomy, Anhui Agricultural University, Hefei 230036, China. Electronic address: weipengcheng@gmail.com.
Haiyang JiangNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China. Electronic address: hyjiang@ahau.edu.cn.
Yijun QiCenter for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: qiyijun@tsinghua.edu.cn.
Pengfei JiangNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China. Electronic address: jiangpengfei@ahau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A class of small RNAs, the phased small interfering RNAs (phasiRNAs), are specifically expressed in anthers and closely associated with male fertility in monocots. However, the specific 21-nt phasiRNAs involved in regulating male fertility remain unclear. Here, we identified a functional 5'-A phasiRNA (referred to as osphasiR1_1) that mediates the cleavage of OsARF7 transcripts to promote male fertility. CRISPR-Cas9 knockout and short tandem target-mimic knockdown of osphasiR1_1 led to significantly reduced male fertility and seed setting. Further mechanistic experiments revealed that osphasiR1_1 mediates OsARF7 cleavage and promotes male fertility in an OsAGO2-dependent but MEL1/OsAGO5c-independent manner. OsARF7 exhibits transcriptional activation activity, and its overexpression hyperactivates target genes such as the key fertility-related gene ubiquitin-fusion ribosomal protein L40 4 and heat shock protein 70-4. Identification of the osphasiR1_1-OsAGO2-OsARF7 regulatory module fills a critical gap in the phasiRNA pathway and expands our understanding of phasiRNA-mediated gene regulation, providing new insight into the molecular mechanisms that control male fertility in monocots.

Indexed as

OryzaPlant ProteinsRNA, Small InterferingFertilityGene Expression Regulation, PlantPlant ProteinsRNA, Small InterferingAGOARFmale fertilityphasiRNAricetranscriptional activation

Identifiers

PMID41782371
PMCPMC13477049

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.