Evidence map›Paper›PMID 41782202›Full record

ArticleFEBS open bio2026

Screening and epitope characterization of Nidogen-2-specific nanobodies.

Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jianchuan WenCollege of Food Science and Technology, Shanghai Ocean University, China.ORCID https://orcid.org/0009-0003-4245-8407
Qianqian CuiShanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Zhongyun LanShanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Yingjun WangCollege of Food Science and Technology, Shanghai Ocean University, China.
Shuaiying ZhaoCollege of Food Science and Technology, Shanghai Ocean University, China.
Wenxuan FengSchool of Pharmacy, Nanjing University of Chinese Medicine, China.
Yunfeng LiuCollege of Food Science and Technology, Shanghai Ocean University, China.
Qiting HuangCollege of Food Science and Technology, Shanghai Ocean University, China.
Dongna ZhangCollege of Food Science and Technology, Shanghai Ocean University, China.
Jianfeng XuCollege of Food Science and Technology, Shanghai Ocean University, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

NID2 is a key component of the BM and plays an important role in ECM organization and tumor-associated microenvironmental remodeling. In this study, full-length recombinant NID2 was used as an antigen to immunize camels, and a nanobody phage display library was constructed from peripheral blood lymphocytes. Using phage display-based screening, nanobodies recognizing distinct epitopes within the NID2 G1G2 and rod-G3 domains were isolated and characterized. Epitope specificity and competition were further analyzed by BLI, allowing the identification of nonoverlapping nanobody pairs. Based on these results, two high-affinity nanobodies were selected to establish a nanobody-based sandwich ELISA for NID2 detection. This assay enabled the detection of recombinant NID2 at concentrations down to 10 ng·mL

Indexed as

Cell Adhesion MoleculesEpitopesSingle-Domain AntibodiesAnimalsCalcium-Binding ProteinsCamelusEnzyme-Linked Immunosorbent AssayHumansPeptide LibraryRecombinant ProteinsCalcium-Binding ProteinsCell Adhesion MoleculesEpitopesNID2 protein, humanPeptide LibraryRecombinant ProteinsSingle-Domain AntibodiesnanobodyNidogen‐2 (NID2)phage displaySandwich ELISA

Identifiers

PMID41782202
PMCPMC13398667

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.