ArticleRadiation oncology (London, England)2026
Local FLASH radiation exhibits a double-edged effect on the mouse immune system.
Article in Radiation oncology (London, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Single dose of 5 Gy can damage erythrocytes and consequently induces lymphocyte depletion in spleen and circulating blood.Frontiers in immunology · 2026Article
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11 authors.
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Abstract
PURPOSE/OBJECTIVE(S): This study aims to investigate the impact of partial blood volume FLASH irradiation on immune system in a mouse leg model. MATERIALS/
methodsThe right hind limbs of C57BL/6 N mice were irradiated in a single fraction with an electron beam using either 50 Gy/s (2 Gy, 5 Gy, 10 Gy, 20 Gy, delivery time 0.04 –0.4 s) or 10 Gy/s (5 Gy, 10 Gy, 15 Gy, 20 Gy, delivery time 0.5 –1.5 s). A total of 90 mice were used in the experiment, with five mice per dose/time group. T lymphocyte subsets (CD3+, CD4+, CD8+, naïve CD4+, and naïve CD8+) were analyzed in peripheral blood and spleen 1 and 4 days post-irradiation by flow cytometry.
resultsThe dose response curves for the subset reduction can be well described by a sigmoid curve for subsets in both peripheral blood and spleen 1 day post-irradiation. Generally, higher dose rate (50 Gy/s) irradiation induced much less subsets depletion than lower dose rate (10 Gy/s) in peripheral blood, consistent with the simulation result that local FLASH radiotherapy may preserve immune cells in peripheral blood. Interestingly, the non-irradiated spleen also displayed substantial subset depletions with an opposite dose rate-dependent behavior. We speculate that radiation-induced red blood cell senescent may be responsible for this interesting phenomenon.
conclusionThe opposing dose rate–dependent responses in peripheral blood and splenic lymphocyte depletion highlight a dual effect of local FLASH irradiation: higher dose rates may protect circulating immune cells, yet may also potentially enhance damage to the lymphocytes in the spleen.
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