Evidence map›Paper›PMID 41781627›Full record

ArticleNature2026

Exapted CRISPR-Cas12f homologues drive RNA-guided transcription.

Florian T Hoffmann, Tanner Wiegand, Adriana I Palmieri, Juniper Glass-Klaiber, Renjian Xiao, Stephen Tang, Hoang C Le, Chance Meers, George D Lampe, Leifu Chang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Florian T HoffmannDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-0301-4259
Tanner WiegandDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-0528-268X
Adriana I PalmieriDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Juniper Glass-KlaiberDepartment of Cellular Physiology and Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0001-9212-725X
Renjian XiaoDepartment of Biological Sciences, Purdue University, West Lafayette, IN, USA.ORCID http://orcid.org/0000-0002-5914-0812
Stephen TangDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0001-5492-9796
Hoang C LeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Chance MeersDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-2554-3987
George D LampeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-2873-2372
Leifu ChangDepartment of Biological Sciences, Purdue University, West Lafayette, IN, USA.ORCID http://orcid.org/0000-0002-6107-5271
Samuel H SternbergDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. shsternberg@gmail.com.ORCID http://orcid.org/0000-0001-8240-9114

Funding

A high-performance and versatile technology for precision microbiome engineeringR01EB031935 · NIBIB · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Samuel Henry Sternberg, Harris H Wang · 2021 to 2026
$3.3M
Molecular basis for CRISPR RNA-guided nucleic acid cleavage and DNA transpositionR01GM138675 · NIGMS · PURDUE UNIVERSITY · PI CHANG, LEIFU · 2020 to 2024
$1.8M
Molecular basis for CRISPR-associated transposons and RNA-guided transcriptional regulationR35GM158248 · NIGMS · PURDUE UNIVERSITY · PI Leifu Chang · 2025 to 2026
$923k
Mechanisms of reverse transcriptase mediated phage resistance in EnterobacteriaceaeF30AI183830 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Stephen Tang · 2024 to 2026
$158k
Howard Hughes Medical InstituteNIAID NIH HHS F30 AI183830NIBIB NIH HHS R01 EB031935NIGMS NIH HHS R01 GM138675NIGMS NIH HHS R35 GM158248
6 · The paper itself

Abstract

Bacterial transcription initiation is a tightly regulated process that canonically relies on sequence-specific promoter recognition by dedicated sigma (σ) factors, leading to functional DNA engagement by RNA polymerase (RNAP)

Indexed as

CRISPR-Associated ProteinsCRISPR-Cas SystemsEscherichia coliRNA, Guide, CRISPR-Cas SystemsTranscription, GeneticEscherichia coli ProteinsGene Expression Regulation, BacterialPromoter Regions, GeneticSigma FactorTranscriptional ActivationTranscription Initiation, GeneticCRISPR-Associated ProteinsEscherichia coli ProteinsRNA, Guide, CRISPR-Cas SystemsSigma Factor

Identifiers

PMID41781627
PMCPMC13472631

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.