Evidence map›Paper›PMID 41781388›Full record

ArticleNature communications2026

Inhibiting Mrt4-rRNA interaction with fumaramidmycin-based derivatives as an antifungal strategy.

Hongxuan Cao, Jie Tu, Jiahui Chen, Bingzhang Chen, Zeyue Huang, Yingjie Wang, Jing Shen, Xiuqi Hu, Jialin Bo, Li Rao and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Hongxuan Cao *State Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Jie Tu *The Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, China.
Jiahui Chen *Department of Natural Product Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Bingzhang Chen *Shenzhen Bay Laboratory, Guangdong, China.
Zeyue Huang *State Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Yingjie Wang *Shenzhen Bay Laboratory, Guangdong, China.
Jing ShenThe Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, China.
Xiuqi HuState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Jialin BoState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Li RaoState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Zheng LiuState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Nokwanda P MakungaDepartment of Botany and Zoology, Stellenbosch University, Stellenbosch, South Africa.
Muhammad Salman HameedState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China.
Chen SuNational Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai, China.
Jian WanState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China. jianwan@ccnu.edu.cn.
Wenqiang ChangDepartment of Natural Product Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, State Key Laboratory of Microbial Technology, Shandong University, Jinan, China. changwenqiang@sdu.edu.cn.ORCID http://orcid.org/0000-0003-0187-9363
Chunquan ShengThe Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, China. shengcq@smmu.edu.cn.
Yanliang RenState Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, China. renyl@ccnu.edu.cn.ORCID http://orcid.org/0000-0001-8565-2152

Funding

National Natural Science Foundation of China (National Science Foundation of China) 22177036National Natural Science Foundation of China (National Science Foundation of China) 22373039National Natural Science Foundation of China (National Science Foundation of China) 22377030National Natural Science Foundation of China (National Science Foundation of China) 82273975National Natural Science Foundation of China (National Science Foundation of China) 82330109National Natural Science Foundation of China (National Science Foundation of China) 92269102
6 · The paper itself

Abstract

The rise of drug resistance and limitations of current antifungal treatments highlight the urgent need for innovative antifungal strategies. Here we present the development of cis-fumaramidmycin-derived analogs inhibiting the interactions of ribosome assembly factor Mrt4 with rRNA to combat fungal infections. Through antifungal screening, we identified a promising lead 20 with strong efficacy against various drug-resistant fungi, including notorious super-fungus Candida auris. A comprehensive approach combining active-and-inactive-based protein profiling (AIBPP), chemical-genetic profiling, and fluorescence polarization revealed that the antifungal activity of 20 is primarily due to selectively inhibiting essential CaMrt4-rRNA interaction by conjointly covalent engaging C96&C189 on CaMrt4 but inactive for HuMrt4-rRNA interaction, thereby disrupting fungal ribosomal assembly. Therapeutic efficacy of 20 in both Galleria mellonella larvae and murine candidiasis models validate this antifungal strategy. Collectively, our studies provide a potential and much needed therapeutic strategy to address the rapidly rising burden of drug-resistant fungal infections.

Indexed as

Antifungal AgentsFungal ProteinsRNA, RibosomalAnimalsCandida aurisCandidiasisDrug Resistance, FungalLarvaMiceMicrobial Sensitivity TestsAntifungal AgentsFungal ProteinsRNA, Ribosomal

Identifiers

PMID41781388
PMCPMC13077063

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.