Protocol for the precise activation of intradental neurons in mice via electrical stimulation.

Shuhao Wan, Elizabeth A Ronan, Aditi Jha, Akash R Gandhi, Brian S C Constantinescu, Kevin P Pipe, Joshua J Emrick

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Shuhao WanDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: shuhwan@umich.edu.

Elizabeth A RonanDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: lizronan@umich.edu.

Aditi JhaDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.

Akash R GandhiDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.

Brian S C ConstantinescuDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.

Kevin P PipeDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Joshua J EmrickDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: jjemrick@umich.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intradental neurons encode tooth sensation, yet identifying them without sacrificing tooth structural integrity remains challenging. Here, we present a protocol for the non-invasive electrical stimulation of intradental neurons with single-molar tooth resolution in anesthetized mice. We describe details for head fixation to access molars. We then provide steps to deliver direct current (DC) pulses to an individual molar while simultaneously monitoring induced current. The protocol can be combined with neural imaging to identify and characterize intradental sensory responses and circuits. For complete details on the use and execution of this protocol, please refer to Ronan et al.

Indexed as

Electric StimulationNeuronsAnimalsMiceMolarCell biologyNeuroscienceSystems biology

Identifiers

PMID41779615

DOI10.1016/j.xpro.2026.104386

PMCPMC12969412

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.