Evidence map›Paper›PMID 41779554›Full record

ArticleMemorias do Instituto Oswaldo Cruz2026

Mitochondrial genomes and phylogeny of Atratus and Educator Group species of the Melanoconion Section of Culex (Melanoconion) (Diptera: Culicidae)

Tatiane Marques Porangaba de Oliveira, Peter Gordon Foster, Ivy Luizi Rodrigues de Sá, Maria Anice Mureb Sallum

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Article in Memorias do Instituto Oswaldo Cruz, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Tatiane Marques Porangaba de OliveiraUniversidade de São Paulo, Faculdade de Saúde Pública, Departamento de Epidemiologia, São Paulo, SP, Brasil.ORCID 0000-0003-0069-6848
Peter Gordon FosterNatural History Museum, Department of Life Sciences, London, United Kingdom.
Ivy Luizi Rodrigues de SáUniversidade de São Paulo, Faculdade de Saúde Pública, Departamento de Epidemiologia, São Paulo, SP, Brasil.
Maria Anice Mureb SallumUniversidade de São Paulo, Faculdade de Saúde Pública, Departamento de Epidemiologia, São Paulo, SP, Brasil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCulex (Melanoconion) species are known to act as vectors for different arboviruses, and little is known about the mitochondrial genome of these species.

objectivesAiming to expand the genetic knowledge of this subgenus, a 12Kb fragment of the mitochondrial genome was sequenced from 23 specimens belonging to the Atratus and Educator Groups of the subgenus Melanoconion of Culex.

methodsThe sequenced specimens were morphologically identified as Culex dunni, Culex ensiformis, Culex theobaldi, Culex trigeminatus, Culex eknomios, Culex zeteki, Culex near commevynenis, Culex angularis, Culex longistriatus, and Culex near vaxus. The reads were assembled with the reference genome of Culex quinquefasciatus and MITOS2 was used for gene annotation. Values of guanine-cytosine (GC) and adenine-thymine (AT) skews, nucleotide diversity, ratio between non-synonymous (Ka) and synonymous (Ks) substitution, and nucleotide composition were calculated, and phylogenetic analysis was performed.

findingsAs in other Culex mitogenomes, the partial mitochondrial genomes include 12 protein coding genes (PCGs), 15 tRNA, and 1 rRNA (rrnL). The PCGs showed no length variation between the species studied. ND5 gene presented one less base in the new sequences, which generated a stop codon and, consequently, a shorter length in relation to the Cx. quinquefasciatus sequence reference. All specimens showed a positive value for AT-skew and negative for GC-skew. Nucleotide diversity varied between 0.00407 and 0.12085. Ka/Ks values ranged from 0.0 to 2.775. MAIN

conclusionsLeucine and Serine were the most abundant amino acids. Phylogenetic analysis suggested three putative species.

Indexed as

CulexGenome, MitochondrialMosquito VectorsPhylogenyAnimalsSequence Analysis, DNA

Identifiers

PMID41779554
PMCPMC12952796

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