Evidence map›Paper›PMID 41779251›Full record

ArticleMolecular neurobiology2026

Role of eCIRP in Mediating Post-ischemia Microglial Phagocytosis via TREM-2 Receptor: Insights from Porcine and Mouse Cellular Models.

Peijuan Li, Zhangping Sun, Yushu Chen, Zhen Fang, Dongping Yu, Ling Wang, Yanbo Ren, Ping Gong

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peijuan Li *Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to, Shandong First Medical University, Jinan, Shandong, China.
Zhangping Sun *Department of Medical Intensive Care Unit (MICU), Zhongshan Hospital Affiliated to Dalian University, Dalian, Liaoning, China.
Yushu Chen *Department of Emergency, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology) , Shenzhen, No. 1017, Dongmen North Road, Luohu District, Guangdong, 518020, China.
Zhen FangGastrointestinal Surgery, Shandong Provincial Hospital Affiliated to, Shandong First Medical University, Jinan, Shandong, China.
Dongping YuDepartment of Emergency, The Second Affiliated Hospital of Dalian Medical University (Dalian Medical University Cancer Hospital), Dalian, Liaoning, China.
Ling WangDepartment of Critical Care Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi City, Guizhou Province, China.
Yanbo RenDepartment of Emergency, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Ping GongDepartment of Emergency, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology) , Shenzhen, No. 1017, Dongmen North Road, Luohu District, Guangdong, 518020, China. gongp828@sina.cn.

Funding

Medical and industrial cooperation project DMU-1&DICP UN202305shenzhen Key Medical Discipline Construction Fund SZXK046Shenzhen Science and Technology Program JCYJ20230807112007014
6 · The paper itself

Abstract

Cerebral ischemia/reperfusion (I/R) injury triggers potent neuroinflammatory responses, in which microglial activation and dysregulated phagocytosis may contribute to neuronal damage. Extracellular cold-inducible RNA-binding protein (eCIRP), a damage-associated molecular pattern, has been implicated in postischemic inflammation; however, its role in regulating microglial phagocytosis remains poorly understood. This study employed a porcine model of cardiac arrest/reperfusion (CA/R) and a mouse cellular model of oxygen-glucose deprivation/reoxygenation (OGD/R) in BV2 microglia and N2a neuron to investigate the role of the eCIRP-TREM2 signaling in microglial activation. We evaluated protein expression and direct interaction between CIRP and TREM2 by Western blotting, immunofluorescence, co-immunoprecipitation, and surface plasmon resonance (SPR), while assessing phagocytic activity by fluorescent microsphere uptake and synaptic protein expression assays. Both CA/R and OGD/R conditions significantly elevated the levels of CIRP expression and activation of the TREM2-DAP12-Syk-ERK1/2 signaling. Treatment with recombinant human CIRP (rhCIRP) further increased the expression of microglial activation markers and enhanced phagocytic activity, accompanied by reducing the expression of synaptic proteins. These effects were partially mitigated by CIRBP or TREM2 silencing, or ERK inhibition. Our findings support a novel hypothesis that eCIRP may bind to TREM2 and activate downstream signaling in microglia, enhancing their phagocytic responses in the process of I/R-induced neuronal injury. The eCIRP-TREM2 axis may thus represent a potential therapeutic target for modulating neuroinflammation and preserving neuronal integrity.

Indexed as

Membrane GlycoproteinsMicrogliaPhagocytosisReceptors, ImmunologicReperfusion InjuryRNA-Binding ProteinsAnimalsCell LineMiceSignal TransductionSwineCirbp protein, mouseMembrane GlycoproteinsReceptors, ImmunologicRNA-Binding ProteinsTrem2 protein, mouseCardiopulmonary resuscitationeCIRPIschemia-reperfusion injuryMicroglialPhagocytosis

Identifiers

PMID41779251

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.