ArticleMethodsX2026
Refining a low-cost dermatophyte sampling method to enhance culture purity in resource-limited clinical settings in Bangladesh.
Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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8 authors.
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Abstract
Dermatophytosis is an emerging public health concern in developing countries, with rising infection rates and persistent disease burden. Reliable isolation of dermatophytes is essential for effective treatment, surveillance, and downstream analyses, yet it remains challenging in low-resource clinical settings. We evaluated three mycological sampling approaches, including one conventional and two modified techniques to improve the recovery of pure dermatophytes while reducing contamination. The modified approaches comprised a heat-assisted aseptic sampling method to limit airborne contamination and a paper-zip transport approach using heat and sterile filter paper sealed in zip-lock bags before inoculation into the culture medium. The outcomes of all sampling approaches were subsequently assessed using conventional culture techniques. A total of 198 clinical specimens from 66 individuals generated 276 culture outcomes. Statistical analysis showed significant differences among methods in recovery of pure isolates and contamination control, with the Paper-Zip method exhibiting the highest selectivity (
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