Structural characterization and interfacial properties of yeast cytoplasmic protein crosslinked by laccase enzyme.

Kelly Light, Lan Liu, Ashraf Ismail, Christophe Blecker, Salwa Karboune

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Article in Food chemistry: X, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Kelly LightMacDonald Campus, McGill University, 21111 Lakeshore Rd, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

Lan LiuMacDonald Campus, McGill University, 21111 Lakeshore Rd, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

Ashraf IsmailMacDonald Campus, McGill University, 21111 Lakeshore Rd, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

Christophe BleckerGembloux Agro-Bio Tech, Pass. des Déportés 2, 5030 Gembloux, Belgium.

Salwa KarbouneMacDonald Campus, McGill University, 21111 Lakeshore Rd, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present work, yeast cytoplasmic protein (YCP) was modified enzymatically by laccase in the presence and absence of ferulic acid (FA) to improve their technofunctional and interfacial properties. The 3 h, 18 h and 24 h reaction times were selected to represent low, medium and high levels of crosslinking based on the proportion of protein >150 kDa. In samples with FA, LC-MS/MS analysis found the greatest prevalence of linkage was in the form of diferulic acid, whereas those without FA had a greater incidence of linkages in the form of dityrosine and isodityrosine. Air/water interface analysis revealed that non-FA modified YCP had quicker adsorption and an enhanced effect on lowering surface tension, whereas FA modified YCP had slower transfer to the interface. Overall, the relationship between the structure and technofunctional properties of laccase-modified YCP was elucidated. Modification with laccase has the potential to further improve the emulsifying capabilities of YCP.

Indexed as

Diferulic acidDityrosineInterfacial propertiesLaccasePotato proteinProtease inhibitorProtein crosslinkingYeast protein

Identifiers

PMID41777633

DOI10.1016/j.fochx.2026.103658

PMCPMC12950425

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LicenceCC BY-NC-ND

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