ArticleMikrochimica acta2026
Core-shell-shell up-conversion nanoparticles-based fluorescence immunochromatographic assay for simultaneous quantitative detection of aflatoxin B
Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A novel dual-channel core-shell-shell up-conversion nanoparticle-based fluorescence immunochromatographic assay (CSS-UCNPs-dICA) combined with multiple-impurity adsorption purification (MIA) pretreatment was designed for the simultaneous and rapid detection of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) in pet food. Capitalizing on the superior luminescent properties of the CSS-UCNPs, the assay exhibited high sensitivity with limits of detection (LODs) of 0.025 ng/mL for AFB1 and 0.25 ng/mL for FB1. This represents a 20-fold sensitivity enhancement compared to colloidal gold-based immunochromatography. In spiking experiments, the assay achieved recoveries ranging from 97.69% − 104.66% for AFB1 and 97.68%–105.26% for FB1 in pet food samples. The generated results were consistent with data from liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses of naturally contaminated pet foods, confirming the superior practicality, rapidness, and simplicity. Thus, the developed CSS-UCNPs-dICA strip is a promising tool for the rapid, on-site, and large-scale screening of AFB1 and FB1 co-contamination in pet food.
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