ArticleJournal of biomedical optics2026
DNA-hybridization on gold nanospheres: a dual-fluorescence investigation of surface loading and strand length effects.
Article in Journal of biomedical optics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Light sources and detectors: applications in nanoscale imaging, sensing, and actuation.Journal of biomedical optics · 2026Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Significance: Gold nanospheres (AuNSs) functionalized with DNA are powerful tools for studying nanoscale biomolecular interactions through fluorescence modulation. Understanding how DNA conformation influences fluorescence is essential for advancing biosensor design. Aim: We examine how DNA strand length and surface loading govern the fluorescence behavior of Cy5-labeled single-stranded DNA (polyA-Cy5) bound to AuNSs and how these properties change upon hybridization with complementary polyT strands. Approach: PolyA-Cy5 strands of different bases were conjugated to AuNSs and analyzed using steady-state and time-resolved fluorescence spectroscopy before and after polyT hybridization. Results: Surface attachment induced strong fluorescence quenching, with intensity varying with strand length due to differences in DNA conformation. Short strands remained rigid and upright, whereas longer strands adopted more flexible geometries. Upon hybridization, longer duplexes exhibited fluorescence enhancement attributed to increased fluorophore-metal spacing as dsDNA becomes more upright. Lifetime measurements supported these conformational changes and suggest a Förster resonance energy transfer (FRET)-based quenching mechanism. Experiments in fetal bovine serum (FBS) confirmed that hybridization-induced enhancement persists in biologically relevant media. Conclusions: The results reveal strand-length and density-dependent conformational dynamics of DNA on AuNSs and establish a robust fluorescence-based method for probing nanoscale assembly. The nanosystem can also be tracked intracellularly, as demonstrated by its detectable signal in fluorescence imaging.
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