Evidence map›Paper›PMID 41772435›Full record

ArticleBMC microbiology2026

Genomic characteristics and risk factor analysis of carbapenem-non-susceptible Pseudomonas aeruginosa causing bacteremia in a tertiary hospital (2014-2023).

Junbin Zhai, Xiaoli Cao, Chang Liu, Jie Zheng, Yan Zhang, Shuo Gao, Xuejing Xu

Abstract read
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In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Junbin ZhaiDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Xiaoli CaoDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Chang LiuDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Jie ZhengDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Yan ZhangDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China.
Shuo GaoDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China. shuoshuo65@163.com.
Xuejing XuDepartment of Clinical Laboratory, Affiliated Hospital of Medical School, Nanjing Drum Tower Hospital, Nanjing University, Nanjing, China. xxuejing@hotmail.com.

Funding

This work was supported by Fundings for Clinical Trials from the Affiliated Drum Tower Hospital, Medical School of Nanjing University 2021-LCYJ-PY-06
6 · The paper itself

Abstract

backgroundCarbapenem-non-susceptible Pseudomonas aeruginosa (CnSPA) bloodstream infections (BSIs) are associated with high mortality and limited treatment options, yet long-term genomic–clinical data from China are scarce.

methodsWe retrospectively analyzed 52 non-duplicate CnSPA isolates causing BSIs from 2014 to 2023 in a tertiary hospital in eastern China. Whole-genome sequencing was used to characterize antimicrobial resistance genes (ARGs), carbapenem resistance–associated mutations, mobile genetic elements, sequence types (STs), serotypes, genetic relationship, and integron structures. Clinical data were integrated to identify risk factors for mortality and polymicrobial BSIs.

resultsCnSPA isolates showed high resistance to imipenem (98.1%) and multiple β-lactams and fluoroquinolones, while remaining susceptible to colistin. Carbapenemase genes were rare, whereas diverse ARGs, frequent oprD and ampC mutations, and widespread co-localization of ARGs with insertion sequences supported a major role for non-carbapenemase mechanisms and mobile genetic elements in resistance dissemination. Thirty-six distinct STs—including high-risk clones ST235, ST244, ST274, and ST357, heterogeneous serotypes and phylogenetic tree indicated substantial genomic diversity. Integron analysis revealed structurally diverse complete integrons, CALINs, and In0 elements in a subset of genomes. Clinically, mortality was 38.5%; prior carbapenem exposure, organ failure, and prolonged mechanical ventilation were independent predictors of death, while long-term catheterization was associated with polymicrobial BSIs.

conclusionsCnSPA BSIs in this setting are driven by multifactorial resistance mechanisms, high-risk clones, and mobile genetic elements, with modifiable clinical factors strongly linked to poor outcomes.

Indexed as

Anti-Bacterial AgentsBacteremiaCarbapenemsPseudomonas aeruginosaPseudomonas InfectionsBacterial Proteinsbeta-LactamasesChinaDrug Resistance, BacterialDrug Resistance, Multiple, BacterialGenome, BacterialHumansIntegronsMicrobial Sensitivity TestsMutationPhylogenyAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasescarbapenemaseCarbapenemsAntimicrobial resistanceBloodstream infectionsCarbapenem resistanceIntegronMobile genetic elementsOprD mutationsPseudomonas aeruginosaRisk factorsWhole genome sequencing

Identifiers

PMID41772435
PMCPMC13059165

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.