Structure, evolution, phylogeny, and analysis of domain-deficient genes in the IQD gene family of Brassica juncea.

Yan Hu, Xinyue Song, Xiaqin Chen, Shanshan Liu, Yuyu Dang, Yalin Qin, Hui Ling, Weishe Hu

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Yan HuCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China. ynwczyh@126.com.

Xinyue SongCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Xiaqin ChenCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Shanshan LiuCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Yuyu DangCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Yalin QinCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Hui LingCollege of Agriculture and biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.

Weishe HuCollege of Agriculture and Biology, Liaocheng University, Liaocheng, 252059, Shandong, China.

Funding

Graduate Research Innovation Project of Hunan University of Humanities, Science and Technology ZSCX2025Y48Hunan Natural Science Foundation Project 2025JJ70318

6 · The paper itself

Abstract

The plant IQD gene family plays crucial roles in abiotic stress response and plant growth regulation. However, the biological functions of IQD genes in Brassica juncea remain largely unknown. Here, we conducted biological analyses to identify and characterize the IQD gene family in B. juncea, providing new insights for future research on the IQD gene family. We identified 107 IQD genes in B. juncea, which are distributed across 18 chromosomes. Collinearity analysis reveals that segmental duplication was the primary mode of replication for IQD genes during evolution. The Ka/Ks ratio indicates that the BjIQD genes underwent strong purifying selection during evolution. GO functional annotation analysis shows that protein binding, tubulin binding, and microtubule binding are the most enriched GO terms. qRT-PCR analysis reveals that the BjIQD genes are regulated in response to Zn stress. Furthermore, some genes lacking the key IQ and DUF4005 domains were analyzed, and we hypothesize that these domain-deficient genes may still perform certain biological functions. This study analyzed the B. juncea IQD gene family from multiple perspectives and explored members lacking key domains. These findings provide insights into the evolution of the BjIQD genes and the analysis of different gene families.

Indexed as

Evolution, MolecularGenes, PlantMultigene FamilyMustard PlantPhylogenyPlant ProteinsGene Expression Regulation, PlantProtein DomainsStress, PhysiologicalPlant ProteinsBrassica junceaEvolutionary analysisExpression analysisIQD gene familyPseudogenesZn stress

Identifiers

PMID41772087

DOI10.1038/s41598-026-42340-2

PMCPMC13065986

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