Aptamer-functionalized magnetic beads combined with hybridization chain reaction for detection of Bacillus cereus.

Xiaoting Song, Changzheng Shi, Yunbin Lv, Xinmei Liu, Xiaomei Bie, Jun Yang

Abstract read

In one paragraph

Article in NPJ science of food, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaoting SongCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.

Changzheng ShiCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.

Yunbin LvCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.

Xinmei LiuKey Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation, Nanjing, China.

Xiaomei BieCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing, China. bxm43@njau.edu.cn.

Jun YangKey Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation, Nanjing, China. yj711003@sina.com.

Funding

Agricultural Independent Innovation Program in Jiangsu Province CX (23)3043Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation KFW202503

6 · The paper itself

Abstract

Based on the specific aptamer-functionalized magnetic beads and the hybrid chain reaction (HCR), a rapid detection method for Bacillus cereus was established. B. cereus competes with carboxylfluorescein-labeled cDNA (FAM-cDNA) to bind to biotin-labeled aptamer fixed on streptavidin magnetic beads. After magnetic separation, the released FAM-cDNA was used as an initiator to trigger HCR amplification, significantly enhancing the fluorescence signal. In the absence of B. cereus, the initiator binds to the aptamer, generating only a weak fluorescent signal. More importantly, graphene oxide (GO) can quench the fluorescence of unreacted FAM-cDNA, achieving a more accurate detection effect and thus enabling the detection of B. cereus at lower concentrations. This method has good specificity and can detect B. cereus as low as 5.4×10

Identifiers

PMID41771875

DOI10.1038/s41538-026-00751-5

PMCPMC13066604

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.