Evidence map›Paper›PMID 41771816›Full record

ArticleChemistry & biodiversity2026

Phytochemical Variability of Mentha L. Species Over Three Growing Seasons.

Helena Pluháčková, Barbora Kudláčková, Marián Šinko, Markéta Michutová, Jarmila Neugebauerová, Lenka Svojanovská

Abstract read
In one paragraph

Article in Chemistry & biodiversity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Helena PluháčkováDepartment of Crop Science, Breeding and Plant Protection, Mendel University in Brno, Brno, Czech Republic.ORCID https://orcid.org/0000-0001-9594-6111
Barbora KudláčkováInstitute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.ORCID https://orcid.org/0000-0002-3181-554X
Marián ŠinkoLandscape Research Institute, Pruhonice, Czech Republic.ORCID https://orcid.org/0009-0000-2877-6722
Markéta MichutováDepartment of Crop Science, Breeding and Plant Protection, Mendel University in Brno, Brno, Czech Republic.ORCID https://orcid.org/0000-0002-3889-9010
Jarmila NeugebauerováDepartment of Vegetable Growing and Floriculture, Mendel University in Brno, Brno, Czech Republic.ORCID https://orcid.org/0000-0003-3582-3325
Lenka SvojanovskáInstitute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.ORCID https://orcid.org/0000-0002-3050-2955

Funding

Institute of Analytical Chemistry of the Czech Academy of Sciences RVO:68081715Research Infrastructure for Young Scientists CZ.02.1.01/0.0/0.0/16_017/0002334
6 · The paper itself

Abstract

Species of the genus Mentha are valued for their aroma and bioactivity. This study presents a comprehensive 3-year (2022-2024) evaluation of two Mentha spicata and three Mentha × piperita genotypes. Methanolic extracts were analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA) using spectrophotometry; individual phenolic compounds were determined by HPLC-DAD, and essential oils (EOs) were characterized by GC-MS. Significant inter- and intra-species variability was observed. M. × piperita showed higher phytochemical levels (TPC: 33.61-55.23 mg GAE/g DW; TFC: 27.56-43.01 mg CE/g DW; and AA: 46.48-103.93 mg TE/g DW) than M. spicata (TPC: 28.92-39.57 mg GAE/g DW; TFC: 23.48-35.11 mg CE/g DW; and AA: 40.09-93.59 mg TE/g DW). Rosmarinic acid predominated in all samples (6227.11 to 29321.70 µg/g DW), with elevated levels observed in M. × piperita. EO yields ranged from 0.55% to 2.31% (v/w), with carvone dominant in M. spicata and menthone, dl-menthol, or piperitenone oxide in M. × piperita. Principal component analysis confirmed distinct chemical differentiation among genotypes. Genotype had a strong, and year a moderate, effect on phytochemical profiles. These results highlight the species-specific potential of Mentha for food, pharmaceutical, and cosmetic applications.

Indexed as

AntioxidantsMenthaPhytochemicalsSeasonsChromatography, High Pressure LiquidFlavonoidsGas Chromatography-Mass SpectrometryOils, VolatilePhenolsPlant ExtractsAntioxidantsFlavonoidsOils, VolatilePhenolsPhytochemicalsPlant Extractsgas chromatographyliquid chromatographyMentha L. speciesphytochemistryUV/Vis spectroscopy

Identifiers

PMID41771816
PMCPMC12953208

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.