ArticleCureus2026
Isolation of Bacteriophages and Their Lytic Profile From River and Waste Water Samples in South Western Uganda.
Article in Cureus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
7 authors.
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Abstract
Background Bacteriophages are found almost everywhere in the environment, such as water, soil, and air, among others. They are effective against a wide range of pathogens because they are highly specific. This study aimed to identify phages from river and wastewater samples in south-western Uganda and determine their lysis profile against selected clinical bacterial isolates. Materials and methods Four different locations (Mbarara city and Ntungamo, Kabale, and Kisoro districts) in south-western Uganda were chosen for the collection of river and wastewater for isolation of potential phages. The culture and double agar overlay method was used to detect the lytic bacteriophages. Isolated phages were purified after diluting them in saline magnesium (SM) buffer, then centrifuged at 3,000 rpm for 10 minutes, and filtered through a 0.22 um syringe filter. After purification, the filtrate was used to determine the phage lytic activity, host range activity (using the spot method), and thermal stability (using the double agar overlay method). Results A total of 17 phages were isolated using
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