Evidence map›Paper›PMID 41768765›Full record

ArticleACS central science2026

Direct Readout of Multivalent Chromatin Reader-Nucleosome Interactions by Nucleosome Mass Spectrometry.

Alexander S Lee, Nickolas P Fisher, Matthew R Marunde, Pei Su, Laiba F Khan, Ryan J Ezell, Zachary B Gillespie, Bria Graham, Hailey F Taylor, Ugochi C Onuoha and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS central science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors.

Alexander S LeeDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.ORCID https://orcid.org/0000-0001-6214-1990
Nickolas P FisherDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.
Matthew R MarundeEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Pei SuDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.ORCID https://orcid.org/0000-0001-6148-0181
Laiba F KhanEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Ryan J EzellEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Zachary B GillespieEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Bria GrahamEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Hailey F TaylorEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Ugochi C OnuohaEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.ORCID https://orcid.org/0009-0002-4132-0054
Taojunfeng SuDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.ORCID https://orcid.org/0000-0002-7111-1248
Kevin JooßDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.
Luis F SchachnerDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.
Harrison A FuchsDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, United States.
Kelsey NollEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Matthew J MeinersEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Marcus A CheekEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Jonathan M BurgEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Zu-Wen SunEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Catherine A MusselmanDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, United States.ORCID https://orcid.org/0000-0002-8356-7971
Michael-Christopher KeoghEpiCypher, Inc., Research Triangle Park, Durham, North Carolina 27709, United States.
Neil L KelleherDepartments of Chemistry and Molecular Biosciences, the Chemistry of Life Processes Institute, and the Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.ORCID https://orcid.org/0000-0002-8815-3372

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Histone post-translational modifications (PTMs) often serve as distinct recognition sites for the recruitment of chromatin-associated proteins (CAPs) for epigenome regulation. While CAP:PTM interactions are extensively studied using histone peptides, this cannot represent the regulatory potential of multisite binding on intact nucleosomes. To overcome this limitation, we applied Nucleosome Mass Spectrometry (Nuc-MS), a native Top-Down MS approach that enables the controlled disassembly and proteoform analysis of CAP:nucleosome (CAP:nuc) complexes. As proof of principle, we show the BPTF plant homeodomain (PHD)-bromodomain (BD) native tandem reader binds synergistically to both PTM classes in fully defined ([H3K4me3K9acK14acK18ac]

Identifiers

PMID41768765
PMCPMC12947556

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.