Evidence map›Paper›PMID 41766436›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

High-Sensitive Mass Cytometry Metal Tags Based on Noble Metal Nanoparticles Assisting Acute Myeloid Leukemia Immune Checkpoint Profiling.

Zhizhou Liu, Yu Yang, Lingfei Qiu, Yi Yang, Jingrui Li, Juan Yue, Yinghui Li, Yingdai Gao, Pengli Bai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhizhou LiuSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Yu YangSchool of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin, China.
Lingfei QiuSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Yi YangSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Jingrui LiSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Juan YueSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
Yinghui LiState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Yingdai GaoState Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Pengli BaiSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.

Funding

Basic Research Pilot Project of Suzhou SSD2023007CAMS Innovation Fund for Medical Science 2025-I2M-XHXX-132Ministry of Science and Technology of the People's Republic of China 2022YFF0710200Natural Science Foundation of Tianjin 23JCZDJC00310Suzhou Basic Research Program-Frontier Technology Research SYG202347Youth Innovation Promotion Association, the Chinese Academy of Sciences Y2023088
6 · The paper itself

Abstract

Mass cytometry is optimal for acute leukemia immune checkpoint profiling. However, current commercial metal-chelating polymer (MCP) metal tags limit its sensitivity due to low metal loading capacity. We report a novel synthetic strategy employing antibody-conjugated gold or platinum nanoparticles (AuNP/PtNP) for high-dimensional single-cell immunophenotyping in both single cell suspension mass cytometry and imaging mass cytometry. Extremely low-dose AuNP (34 NPs/cell) exhibited MCP-comparable sensitivity, while high doses enabled up to 48-fold signal amplification. Furthermore, integration of the detection of low-abundance TIGIT and OX40 by AuNP/PtNP tags and other 22 biomarkers by MCP tags, three significantly distinct T cell subpopulations in healthy donor and acute myeloid leukemia samples were identified, providing new targets and intervention strategies for the diagnosis and treatment of acute myeloid leukemia. The proposed strategies demonstrate that noble metal NP-based tags show promising potential in detection of low-abundance cell surface biomarkers and enhance MC implementation in both biomedical and clinical applications.

Indexed as

Flow CytometryImmunophenotypingLeukemia, Myeloid, AcuteMetal NanoparticlesGoldHumansSingle-Cell AnalysisGoldAML immune checkpoint profilingmass cytometrynoble metal nanoparticlessingle cell analysis

Identifiers

PMID41766436
PMCPMC13137793

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.