Evidence map›Paper›PMID 41765877›Full record

ArticleBMC genomics2026

Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.

Tiancang Han, Zhichao Wang, Xinqi Gan, Zhiyuan Yang, Wenzhuo Wang, Kunlong Hu, Ruiqing Zhu, Yunsheng Li, Yunhai Zhang, Zubing Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tiancang Han *Anhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Zhichao Wang *Anhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Xinqi GanAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Zhiyuan YangAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Wenzhuo WangAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Kunlong HuAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Ruiqing ZhuAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Yunsheng LiAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China.
Yunhai ZhangAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China. yunhaizhang@ahau.edu.cn.
Zubing CaoAnhui Province Key Laboratory of Local Livestock and Poultry, Genetic Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road,, Hefei, 230036, China. zubingcao@ahau.edu.cn.

Funding

the Anhui Province Higher Education Institutions Scientific Research Project Scientific Research Projects of Universities in Anhui 2024AH040078the Anhui Provincial Science and Technology Commissioner Project 202513b10050016the National Key Research and Development Program of China 2023YFD1300502-1
6 · The paper itself

Abstract

backgroundBovine oocyte quality is crucial for successful in vitro embryo production; therefore, elucidating the mechanisms underlying oocyte maturation is essential. N6-methyladenosine (m6A) modification plays a pivotal role in gametogenesis and embryonic development. However, the dynamic landscape and functional significance of m6A modification during bovine oocyte maturation remain poorly understood.

resultsWe performed transcriptome-wide m6A profiling analysis on bovine oocytes at the GV and MII stages using MeRIP-seq and RNA-seq. A total of 6323 differential m6A peaks were identified between the two groups, with 4422 significantly upregulated and 1901 significantly downregulated. The combined analysis of MeRIP-seq and RNA-seq identified 262 genes with significant differences in both methylation modification and transcriptome levels. GO and KEGG enrichment analyses of these genes indicated that they were significantly enriched in biological processes and signaling pathways, including endocytosis, thermogenesis, ribosome biogenesis, cellular metabolism, the Apelin signaling pathway, and the HIF-1 signaling pathway. Finally, we assessed the roles of m6A modification in bovine oocyte maturation. The results demonstrated that inhibition of m6A modification impairs both nuclear and cytoplasmic maturation of bovine oocytes.

conclusionsOur results provide a comprehensive m6A modification profile during bovine oocyte maturation. Moreover, we found that inhibition of m6A modification results in meiotic arrest and impaired oocyte maturation. These findings offer a theoretical foundation for further studies on the function of RNA modifications in oocyte maturation.

Indexed as

AdenosineOocytesAnimalsCattleEpitranscriptomeEpitranscriptomicsFemaleGene Expression ProfilingRNA MethylationTranscriptomeAdenosineN-methyladenosineBovineIn vitro maturationN6-methyladenosineOocyte

Identifiers

PMID41765877
PMCPMC13059502

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.