Isolation and characterization of a lytic bacteriophage against Staphylococcus aureus.

Yi Liu, Minfeng Ding, Tiande Zou, Xingping Chen, Xin Li, Yan Chen, Jun Chen, Jinming You

Abstract read

In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Yi LiuJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Minfeng DingJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Tiande ZouJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Xingping ChenJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Xin LiJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Yan ChenJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China.

Jun ChenJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China. junchen@jxau.edu.cn.

Jinming YouJiangxi Province Key Laboratory of Animal Nutrition and Feed, Jiangxi Province Key Innovation Center of Integration in Production and Education for High Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang, 330045, China. youjinm@163.com.

Funding

Jiangxi Provincial Cultivation Program for Academic and Technical Leaders of Major Subjects No. 20213BCJ22005Key Research and Development Program of Jiangxi Province No. 20223BBF61018 and No. 20224BBF61029National Natural Science Foundation of China No. 32472940 and No. 32160806

6 · The paper itself

Abstract

Staphylococcus aureus (S. aureus) is one of major pathogens in animal production. S. aureus-induced mastitis poses a serious threat to animal health and leads to significant economic losses. The objective of this study was to isolate and characterize bacteriophage(s) specific to S. aureus and assess its (their) in vitro antibacterial activity. A strain of S. aureus bacteriophage, designated JXAU-SA-P1, was isolated from sewage in a commercial pig farm. Morphological analysis revealed that JXAU-SA-P1 belongs to the Caudovimetes class. The genome of JXAU-SA-P1 is 134.23 kb in length with a GC content of 29.99%, and no virulence or antibiotic resistance genes were identified. JXAU-SA-P1 exhibited a low optimal multiplicity of infection (0.001), a high burst size (300 PFU/cell), and significant antibacterial activity. The bacteriophage showed stable activity below 50 °C and within a pH range of 2.0–12.0. JXAU-SA-P1 effectively inhibited the growth of S. aureus in milk at both 4 °C and 25 °C. Furthermore, it demonstrated substantial biofilm prevention and removal properties. In conclusion, these findings indicate that JXAU-SA-P1 is a potential antibacterial agent for the control of S. aureus-induced mastitis.

Indexed as

Staphylococcus aureusStaphylococcus PhagesAnimalsBiofilmsFemaleMilkSewageStaphylococcal InfectionsSwineSewageBacteriophageBiological propertiesCharacterizationIsolationStaphylococcus aureus

Identifiers

PMID41764498

DOI10.1186/s12917-026-05371-5

PMCPMC13059448

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.