Evidence map›Paper›PMID 41764388›Full record

ArticleJournal of water and health2026

Proof-of-concept of host attribution of antimicrobial resistance genes using wastewater Hi-C metagenome sequencing.

Kongyang Zhu, Ayaaz Amirali, Benjamin Auch, Kristina M Babler, Pratim Biswas, Kate Bowie, Shruti Choudhary, Benjamin B Currall, George S Grills, Hannah G Healy and 12 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of water and health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Kongyang ZhuDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Ayaaz AmiraliDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Benjamin AuchPhase Genomics, Seattle, WA 98109, USA.
Kristina M BablerDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Pratim BiswasDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Kate BowieDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Shruti ChoudharyDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Benjamin B CurrallSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
George S GrillsSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Hannah G HealyDepartment of Environmental Health, T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA.
Ivan LiachkoPhase Genomics, Seattle, WA 98109, USA.
Alexander G LucaciDepartment of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA.
Christopher E MasonDepartment of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA.
Mark SharkeyDepartment of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Oona Shigeno Risse-AdamsDepartment of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA.
Bhavarth S ShuklaDepartment of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Zach SissonPhase Genomics, Seattle, WA 98109, USA.
Mario StevensonDepartment of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Sion L WilliamsSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Alessandro ZulliDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Jordan PecciaDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Helena M Solo-GabrieleDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA E-mail: hmsolo@miami.edu.ORCID https://orcid.org/0000-0003-3390-3823

Funding

University of Miami Developmental Center for AIDS Research (D-CFAR)P30AI073961 · NIAID · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Savita Pahwa · 2007 to 2026
$33.8M
Supplement for MINI point-of-use deviceU01DA053941 · NIDA · UNIVERSITY OF MIAMI CORAL GABLES · PI MASON, CHRISTOPHER EDWARD, SCHURER, STEPHAN C · 2021 to 2022
$5.2M
4CatalyzerNIDA NIH HHS U01DA053941NIH HHS P30AI073961
6 · The paper itself

Abstract

The proliferation of antimicrobial resistance genes (ARGs) poses public health risks globally, with wastewater treatment plants (WWTPs) serving as dissemination hubs for horizontal gene transfer. In this study, we evaluated the potential of applying Hi-C sequencing coupled with metagenomic bioinformatics for surveillance of ARGs and other microbial fitness traits using samples from WWTPs. Hi-C sequencing has the advantage over other molecular approaches by directly associating genes conveying fitness to their host microbe, plus to their element type (in plasmids, phages, or within the core genome of its host microbe). Results from Hi-C analyses confirm results from more laborious approaches by showing that aminoglycoside resistance is disseminated by plasmids. Mercury resistance was found in

Indexed as

BacteriaDrug Resistance, BacterialDrug Resistance, MicrobialGenes, BacterialMetagenomeWastewaterWastewateraminoglycosideantimicrobial resistance genes (ARG)high-throughput chromosome conformation capture (Hi-C)quaternary ammonium compounds (QACs)stress genesvirulence genes

Identifiers

PMID41764388

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.