Decoding mycobacterial ecology in Sub-Saharan African wastewater: metagenomic and metatranscriptomic insights for tuberculosis surveillance.

Hlengiwe N Mtetwa, Isaac D Amoah, Nonsikelelo P Mthethwa-Hlongwa, Rian Pieerneef, Sheena Kumari, Faizal Bux, Poovendhree Reddy

Abstract read

PubMed Publisher

In one paragraph

Article in Journal of water and health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hlengiwe N MtetwaInstitute for Water and Wastewater Technology (IWWT), Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa.ORCID https://orcid.org/0000-0002-4184-204X

Isaac D AmoahDepartment of Environmental Science, University of Arizona, Tucson, AZ 85721, Usa.ORCID https://orcid.org/0000-0002-0131-3442

Nonsikelelo P Mthethwa-HlongwaStellenbosch University, Microbiology, Faculty of Science, School for Data Science and Computational Thinking, Stellenbosch, 7600, South Africa.ORCID https://orcid.org/0000-0002-4800-4006

Rian PieerneefCentre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, 0001, South Africa.ORCID https://orcid.org/0000-0002-0619-0058

Sheena KumariInstitute for Water and Wastewater Technology (IWWT), Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa.ORCID https://orcid.org/0000-0002-3436-8263

Faizal BuxInstitute for Water and Wastewater Technology (IWWT), Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa.

Poovendhree ReddyInstitute for Water and Wastewater Technology (IWWT), Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa.ORCID https://orcid.org/0000-0003-0197-8951

Funding

South African Medical Research Council 96086

6 · The paper itself

Abstract

Tuberculosis (TB) remains a major public health challenge in sub-Saharan Africa, driven by high transmission, delayed diagnosis, and limited surveillance. This study presents one of the first integrated applications of shotgun metagenomic and metatranscriptomic sequencing to investigate

Indexed as

MetagenomeMycobacteriumTuberculosisWastewaterAfrica South of the SaharaAnimalsHumansMetagenomicsWastewaterepidemiology and AfricametagenomicsmetatranscriptomicsTuberculosiswastewater

Identifiers

PMID41764386

DOI10.2166/wh.2026.129

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.