Evidence map›Paper›PMID 41762654›Full record

ArticleSTAR protocols2026

Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound screening.

Payal Chawla, Niclas Flosdorf, Marcelo A S de Toledo, Martin Zenke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Payal ChawlaDepartment of Medicine IV, Hematology, Oncology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Hospital, 52074 Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), 52074 Aachen, Germany.
Niclas FlosdorfInstitute for Cell and Tumor Biology, RWTH Aachen University Medical School, 52074 Aachen, Germany.
Marcelo A S de ToledoDepartment of Medicine IV, Hematology, Oncology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Hospital, 52074 Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), 52074 Aachen, Germany.
Martin ZenkeDepartment of Medicine IV, Hematology, Oncology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Hospital, 52074 Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), 52074 Aachen, Germany. Electronic address: martin.zenke@rwth-aachen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for generating megakaryocytes from patient induced pluripotent stem cells (iPSCs). We describe steps for expanding and dissociating iPSCs into single cells and aggregating them into spin embryoid bodies (EBs). We detail procedures for lineage commitment and differentiation toward megakaryocytes within 14 days. This protocol supports phenotypic characterization and compound testing across multiple patient-derived iPSC lines. For complete details on the use and execution of this protocol, please refer to Flosdorf et al.

Indexed as

Cell Culture TechniquesInduced Pluripotent Stem CellsMegakaryocytesCell DifferentiationEmbryoid BodiesHumansCell-based AssaysCell BiologyCell cultureCell DifferentiationFlow CytometryStem Cells

Identifiers

PMID41762654
PMCPMC12964004

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.