ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2026
Gene mapping and identification of candidate genes associated with fomesafen herbicide tolerance in Mung bean (Vigna radiata L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
key messageThrough an integrated approach of genetic mapping, transcriptomics, and functional validation, we identified VrGSTU18 as the primary gene associated with fomesafen resistance in mung bean, providing a genetic resource for breeding herbicide-resistant varieties. Herbicides are widely applied for weed control in mung bean cultivation, and the development of new varieties with herbicide resistance is critical for weed management. In this study, a recombinant inbred line (RIL) population, derived from a cross between the fomesafen-resistant variety LZ177 and susceptible variety LD235, was used to map the genes related to fomesafen herbicide resistance. Genetic segregation analysis indicated that fomesafen resistance is controlled by a single dominant gene, following a 3:1 ratio. Genetic mapping combined BSA-seq revealed a candidate region of 1.17 Mb on chromosome 11. RNA-seq analysis of residual heterozygous line 198-comparing resistant (RHL198-R) and susceptible (RHL198-S) bulks at 0, 12, 24, 48, and 72 h after fomesafen treatment-identified 14,402 herbicide-responsive genes. Weighted gene coexpression network analysis (WGCNA) further identified nine modules highly correlated with fomesafen resistance, of which 13 potential candidate genes were selected within the 1.17 Mb interval. Among these, one-base (A) insertion/deletion in the exon of jg37117, which encode a tau-class glutathione S-transferase U18 (GSTU18), emerged as the most promising candidate gene. Heterologous expression of VrGSTU18 cloned from LZ177 in Arabidopsis conferred enhanced fomesafen resistance in T
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