ArticleCurrent protocols2026
Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi-Marker Amplicon Panel.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi-Marker Amplicon Panel.Current protocols · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Schistosomes are parasitic trematode worms of the genus Schistosoma, responsible for causing urogenital and intestinal schistosomiasis. Six primary species infect humans: S. mansoni, S. japonicum, S. mekongi, S. intercalatum, S. guineensis, and S. haematobium. In addition, several species including S. bovis, S. curassoni, and S. mattheei primarily infect animals, particularly cattle. Hybridization events have been documented both between human-infecting species and between human- and animal-infecting species of Schistosoma. Current methods for detecting hybrids rely on genotyping mitochondrial cytochrome oxidase I (cox1) and the ribosomal internal transcribed spacer (ITS), or whole-genome sequencing. This protocol describes a multi-locus, multiplex amplicon sequencing approach using Nanopore technology, referred to as NMAS-Seq, for species-level genotyping and hybrid detection. It includes comprehensive steps for multiplex nested PCR, amplicon quantification and pooling, library preparation, and sequencing. The workflow is adaptable for molecular typing of other parasitic species. © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: DNA extraction and multiplex nested PCR Basic Protocol 2: Amplicon quantification and pooling Basic Protocol 3: Amplicon library preparation and Nanopore sequencing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.